Construct: ORF ccsbBroad304_12594
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014916.1_s304c1, BRDN0000400417
- Derived from:
- ccsbBroadEn_12594
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CENPU (79682)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12594
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 79682 | CENPU | centromere protein U | NM_024629.4 | 42.1% | 42.1% | 1_726del |
| 2 | human | 79682 | CENPU | centromere protein U | XM_005263218.4 | 39.2% | 39.2% | 1_816del |
| 3 | human | 79682 | CENPU | centromere protein U | NR_104593.2 | 18.6% | 1_760del;957_958ins62;1227_2432del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 594
- ORF length:
- 528
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa aaccagtgac atcaaggagt tgaatattgt tttgcctgaa tttgagaaaa 121 cccacctaga gcatcaacaa agaatagaat ctaaagtttg taaggcagcc atcgccacat 181 tttatgttaa tgttaaagaa caattcatca aaatgcttaa agaaagccag atgttgacaa 241 atctgaaaag gaagaatgct aagatgattt cagatatcga aaagaaaagg cagcgtatga 301 ttgaagtcca ggatgaactg cttcggttag agccacagct gaaacaacta caaacaaaat 361 atgatgaact taaagagaga aagtcttccc ttaggaatgc agcatatttc ttatctaatt 421 taaaacagct ttatcaagat tattcagatg ttcaagctca agaaccaaac gtaaaggaaa 481 cgtatgattc atccagcctt ccagctctgt tatttaaagc aagaacactt ctgggagccg 541 aaagccatct gcgaaatatc aaccatcagt tagagaagct ccttgaccag ggatgcccaa 601 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 661 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta