Construct: ORF ccsbBroad304_12634

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016774.1_s304c1, BRDN0000404094
Derived from:
ccsbBroadEn_12634
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
CSPP1 (79848)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_12634
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 79848 CSPP1 centrosome and spindle pole... XM_017013856.2 20.6% 20.4% 616_617delAA;627_3024del
2 human 79848 CSPP1 centrosome and spindle pole... XM_024447282.1 20% 19.9% 302_382del;697_698delAA;708_3105del
3 human 79848 CSPP1 centrosome and spindle pole... XM_017013855.2 20% 19.8% 1_246del;862_863delAA;873_3117del
4 human 79848 CSPP1 centrosome and spindle pole... XM_017013854.2 18.6% 18.4% (many diffs)
5 human 79848 CSPP1 centrosome and spindle pole... XM_006716477.3 18.4% 18.3% (many diffs)
6 human 79848 CSPP1 centrosome and spindle pole... NM_001363133.2 18.3% 18.1% 616_617delAA;627_3402del
7 human 79848 CSPP1 centrosome and spindle pole... XM_024447281.1 18.3% 18.1% 616_617delAA;627_3402del
8 human 79848 CSPP1 centrosome and spindle pole... NM_001363132.2 17.9% 17.7% 302_382del;697_698delAA;708_3483del
9 human 79848 CSPP1 centrosome and spindle pole... XM_017013852.2 17.5% 17.4% 1_246del;862_863delAA;873_3549del
10 human 79848 CSPP1 centrosome and spindle pole... XM_017013851.2 17.5% 17.3% 616_617delAA;627_3555del
11 human 79848 CSPP1 centrosome and spindle pole... NM_001364870.1 17.5% 17.3% 1_108del;724_725delAA;735_3564del
12 human 79848 CSPP1 centrosome and spindle pole... NM_001363131.2 17.3% 17.1% 616_617delAA;627_3597del
13 human 79848 CSPP1 centrosome and spindle pole... XM_024447279.1 17.3% 17.1% 616_617delAA;627_3597del
14 human 79848 CSPP1 centrosome and spindle pole... NM_024790.6 17% 16.8% 1_108del;724_725delAA;735_3663del
15 human 79848 CSPP1 centrosome and spindle pole... XM_011517607.2 16.9% 16.8% 302_382del;697_698delAA;708_3678del
16 human 79848 CSPP1 centrosome and spindle pole... XM_024447278.1 16.9% 16.8% 302_382del;697_698delAA;708_3678del
17 human 79848 CSPP1 centrosome and spindle pole... XM_011517603.2 16.8% 16.6% 302_406del;721_722delAA;732_3702del
18 human 79848 CSPP1 centrosome and spindle pole... XM_017013850.2 16.8% 16.6% 1_246del;862_863delAA;873_3702del
19 human 79848 CSPP1 centrosome and spindle pole... NM_001364869.1 16.6% 16.5% (many diffs)
20 human 79848 CSPP1 centrosome and spindle pole... XM_017013849.2 16.6% 16.5% 1_246del;862_863delAA;873_3744del
21 human 79848 CSPP1 centrosome and spindle pole... XM_006716474.3 16.6% 16.4% (many diffs)
22 human 79848 CSPP1 centrosome and spindle pole... XM_017013848.2 16.4% 16.3% (many diffs)
23 human 79848 CSPP1 centrosome and spindle pole... XM_011517602.2 16.4% 16.2% 1_246del;862_863delAA;873_3801del
24 human 79848 CSPP1 centrosome and spindle pole... XM_017013847.2 16.3% 16.2% (many diffs)
25 human 79848 CSPP1 centrosome and spindle pole... XM_011517601.2 16.2% 16% 1_246del;862_863delAA;873_3843del
26 human 79848 CSPP1 centrosome and spindle pole... XM_011517600.2 16% 15.9% (many diffs)
27 human 79848 CSPP1 centrosome and spindle pole... XM_005251305.4 15.9% 15.8% (many diffs)
28 human 79848 CSPP1 centrosome and spindle pole... XM_011517599.2 15.9% 15.7% (many diffs)
29 human 79848 CSPP1 centrosome and spindle pole... XM_011517598.2 15.8% 15.6% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
690
ORF length:
624
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc tgataatttg gatgaattta ttgaagagca aaaagccaga ttggccgaag
121acaaagcaga gttggaaagt gatccacctt acatggaaat gaagggaaag ttgtcagcga
181agctttctga aaacagtaag atactgatct ctatggctaa ggaaaacata ccaccaaata
241gtcaacagac caggggttcc ttaggaattg attatggatt aagtttacca cttggagaag
301actatgaacg gaagaaacat aaattaaaag aagaattgcg gcaagattac agacgttatc
361ttactcagga aaggttgaaa cttgaacgta acaaagaata caatcagttt ctcaggggta
421aggaagaatc cagtgaaaag ttcaggcagg tggaaaagag tactgagccc aagagtcaga
481gaaataaaaa acctattggt caagttaagc ctgatctaac ttcacaaata cagacatctt
541gtgaaaattc agagggtcct agaaaagatg tcttaactcc ttcagaggca tatgaagaac
601ttctgaacca aagacgacta gaggaggaca gataccgaca actagatgat gaaatcgaat
661taaggaatag aagaattatt aaaagcaaat gcccaacttt cttgtacaaa gtggttggta
721agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct agcgctaacc
781ggtggcgcgt taagtcgaca atcaacctct ggatta

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