Construct: ORF ccsbBroad304_12661
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004420.1_s304c1, BRDN0000409082
- Derived from:
- ccsbBroadEn_12661
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DGLUCY (80017)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12661
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001286473.2 | 100% | 100% | |
| 2 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001102369.2 | 25% | 18.3% | (many diffs) |
| 3 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001286472.1 | 24.2% | 17.9% | (many diffs) |
| 4 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001102366.2 | 22.8% | 15.6% | (many diffs) |
| 5 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001102367.2 | 22.8% | 15.6% | (many diffs) |
| 6 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001286471.1 | 22.8% | 15.6% | (many diffs) |
| 7 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001358311.1 | 22.8% | 15.6% | (many diffs) |
| 8 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_024952.7 | 22.8% | 15.6% | (many diffs) |
| 9 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001102368.2 | 22.7% | 16.7% | (many diffs) |
| 10 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001286470.1 | 22.7% | 16.7% | (many diffs) |
| 11 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001358310.1 | 22.7% | 16.7% | (many diffs) |
| 12 | human | 80017 | DGLUCY | D-glutamate cyclase | NM_001358312.1 | 22.7% | 16.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 543
- ORF length:
- 477
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc cttcacactc cacctgaggt cccgccttcc ctctgccata aggagtttga 121 ttctacaaaa gaaaccaaac atcagaaata catccagcat ggctggagag ctccgaccag 181 ccagcctggt ggtcctgccc aggtcccttg ctccagcttt tgaaagattc tgccaggtca 241 acactggtcc tctacccctg ctgggccaga gtgagccaga aaagtggatg ctgccccctc 301 aaggtgctat ctcagagacc agcctcaggg accttcacag tgcctggcac agaacctcac 361 tcatgactgt tgctcaggaa cgagctgccc tccccactgt tctgactcct tcccctatca 421 cctggcgcct gtcctggctg agatctttga gatcatggag aaagtccagc cctggagaaa 481 tcccactggc tctgccatct gtccactcca tcgcccaagc cagaaacccg cctgtacagg 541 attacccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 601 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 661 tctggatta