Construct: ORF ccsbBroad304_12680
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007302.1_s304c1, BRDN0000408077
- Derived from:
- ccsbBroadEn_12680
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- RUBCNL (80183)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12680
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_001286762.3 | 50.5% | 48.9% | 924_984del;1025_1905del |
| 2 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_001286761.2 | 48.4% | 46.9% | 924_984del;1025_1986del |
| 3 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_001349772.2 | 48.4% | 46.9% | 924_984del;1025_1986del |
| 4 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_025113.4 | 48.4% | 46.9% | 924_984del;1025_1986del |
| 5 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_001286763.3 | 37% | 33.8% | (many diffs) |
| 6 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_001286764.3 | 28% | 26.6% | 0_1ins405;519_579del;620_1581del |
| 7 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_001286765.2 | 23.6% | 20.2% | (many diffs) |
| 8 | human | 80183 | RUBCNL | rubicon like autophagy enha... | NM_001286766.2 | 16% | 14.6% | 0_1ins645;279_339del;380_1341del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 61
- ORF end:
- 1024
- ORF length:
- 963
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 atggtgtcac aatctacagt caggcaggat tctcctgtgg agccctggga agggatcagc 121 gatcactctg gcattattga tggttcgccc agactcctga acactgacca tcctccttgc 181 caattagaca tcaggctcat gaggcacaaa gctgtctgga ttaaccccca ggatgtgcag 241 caacagccgc aggacttgca atctcaggtg ccagcagcag ggaacagtgg gacccatttt 301 gtgacagatg ctgcctctcc ctcaggccct tcaccttcgt gcctcgggga ctccctggca 361 gagacaacgt tgtctgagga taccacagac tccgttggca gcgcttctcc ccatggctcg 421 agtgaaaaga gtagcagctt ctctctgtcc tcaacagagg tacacatggt ccgcccagga 481 tactctcatc gggtgtctct gcccacaagc cctgggattt tggccacctc cccatatcct 541 gagactgaca gtgctttttt tgagccttcc catctgacat ctgctgctga tgaaggtgct 601 gttcaagtca gtagaagaac catttcttcg aattccttct caccagaggt atttgtgctg 661 cctgttgatg tagaaaagga aaatgcccac ttttatgttg cagatatgat tatatcagca 721 atggagaaaa tgaagtgtaa cattctgagt caacagcaga cagagagctg gagtaaagaa 781 gtcagtgggt tacttgggag tgatcagcct gactctgaaa tgacttttga taccaacata 841 aagcaagagt ctgggtcttc tacttcttca tacagtggct atgaaggttg tgctgtgtta 901 caggtcagcc cagtgactga aacacgtact taccatgatg tgaaagagat ttgcaaatgc 961 gatgttgatg aatttgttat tttagtgtca cttatgagcc agacttcaat tctgcagaac 1021 tattagccaa atttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt 1081 ctcgattcta cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac 1141 ctctggatta