Construct: ORF ccsbBroad304_12740
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016751.1_s304c1, BRDN0000406698
- Derived from:
- ccsbBroadEn_12740
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CHST9 (83539)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12740
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 83539 | CHST9 | carbohydrate sulfotransfera... | NM_031422.6 | 98.8% | 98.8% | 1_15del |
| 2 | human | 83539 | CHST9 | carbohydrate sulfotransfera... | XM_017026033.1 | 98.8% | 98.8% | 1_15del |
| 3 | human | 83539 | CHST9 | carbohydrate sulfotransfera... | XM_017026034.1 | 87.2% | 86% | (many diffs) |
| 4 | human | 83539 | CHST9 | carbohydrate sulfotransfera... | XM_005258362.4 | 81.7% | 81.7% | 0_1ins240 |
| 5 | human | 83539 | CHST9 | carbohydrate sulfotransfera... | XM_006722555.4 | 20% | 18.5% | (many diffs) |
| 6 | human | 83539 | CHST9 | carbohydrate sulfotransfera... | NM_001256316.2 | 15.5% | 14.6% | 1_15del;202_203ins38;222_223ins1069 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1380
- ORF length:
- 1314
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt catgaacccc aaacaagtct tcctctctgt gctgatattt ggagtagctg 121 ggctactcct cttcatgtat ttgcaagtct ggattgaaga acaacataca gggagagtgg 181 agaagagaag agaacaaaaa gtaacttcag gatggggacc agtgaagtac ttgcggcctg 241 tacccagaat catgagtaca gaaaaaatcc aggaacatat caccaaccag aaccccaagt 301 ttcacatgcc tgaggatgta cgagaaaaaa aggaaaatct tctactcaat tctgagagat 361 ctactaggct cttaacaaag accagtcatt cacaaggagg ggatcaagct ttaagtaagt 421 ccacagggtc accaacagag aagttgattg aaaaacgtca aggagctaag actgttttta 481 acaagttcag caacatgaat tggccagtgg acattcaccc tttaaacaaa agtttagtca 541 aagataataa atggaagaaa actgaggaga cccaagagaa acgaaggtct ttccttcagg 601 agttttgcaa gaaatacggt ggggtgagtc atcatcagtc acatcttttt catacagtat 661 ccagaatcta tgtagaagat aaacacaaaa tcttatattg tgaggtacct aaggctggct 721 gttccaattg gaaaagaatt ctgatggtac taaatggatt ggcttcctct gcatacaaca 781 tctcccacaa tgctgtccac tacgggaagc atttgaagaa gctagatagc tttgacctaa 841 aagggatata tacccgctta aatacttaca ccaaagctgt gtttgttcgt gatcccatgg 901 aaagattagt atcagccttt agggacaaat ttgaacaccc caatagttat taccatccag 961 tattcggaaa ggcaattatc aagaaatatc gaccaaatgc ctgtgaagaa gcattaatta 1021 atggatctgg agtcaagttc aaagagttta tccactactt gctggattcc caccgtccag 1081 taggaatgga cattcactgg gaaaaggtca gcaaactctg ctatccgtgt ttgatcaact 1141 atgattttgt agggaaattt gagactttgg aagaagatgc caattacttt ttacagatga 1201 tcggtgctcc aaaggagctg aaatttccca actttaagga taggcactct tccgatgaaa 1261 gaaccaatgc tcaagtcgtg agacagtatt taaaggatct gactagaact gagagacaat 1321 taatctatga cttttattac ttggactatt taatgtttaa ttatacaact ccatttttgt 1381 acccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 1441 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 1501 ggatta