Construct: ORF ccsbBroad304_12746
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003735.1_s304c1, BRDN0000407318
- Derived from:
- ccsbBroadEn_12746
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- GUCD1 (83606)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12746
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 105371063 | LOC105371063 | putative uncharacterized pr... | XM_011522781.1 | 34.4% | 11.2% | (many diffs) |
| 2 | human | 83606 | GUCD1 | guanylyl cyclase domain con... | NR_104286.2 | 7.6% | 1_2138del;2385_3201del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 312
- ORF length:
- 246
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgcc accatgcccg gctaattttt ttgtattttt agtagagacc aggcttcgcc 121 atgttggcca ggctggtctc gaacccctga gctcaggtga tccacctgcc tcggcctccc 181 aaagtgggaa atgggtcatt ttcaaagggg caacagtgat accatccctg cactcctggg 241 tcctgctctt ccaatgccca aggcatggca tggtggcagc tgtgcccccg cccattgctt 301 tggagcacag atgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 361 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 421 caatcaacct ctggatta