Construct: ORF ccsbBroad304_12878
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002501.1_s304c1, BRDN0000404898
- Derived from:
- ccsbBroadEn_12878
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NFATC2IP (84901)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12878
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84901 | NFATC2IP | nuclear factor of activated... | NM_032815.3 | 30.3% | 30.3% | 1_876del |
| 2 | human | 84901 | NFATC2IP | nuclear factor of activated... | XR_950864.3 | 14.7% | 1_628del;1010_2585del | |
| 3 | mouse | 18020 | Nfatc2ip | nuclear factor of activated... | NM_010900.3 | 26.6% | 27.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 447
- ORF length:
- 381
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc cacccacctt ggggtgtccc caagcaggat ccttttgctt tttggagaga 121 cagagctatc acctactgcc actcccagga ccctaaagct cggagtggct gacatcattg 181 actgtgtggt actaacaagt tctccagagg ccacagagac gtcccaacag ctccagctcc 241 gggtgcaggg aaaggagaaa caccagacac tggaagtctc actgtctcga gattcccctc 301 taaagaccct catgtcccac tatgaggagg ccatgggact gtcgggacgg aagctctcct 361 tcttctttga tgggacaaag ctttcaggca gggagctgcc agctgacctg ggcatggaat 421 ctggggacct cattgaggtc tggggctgcc caactttctt gtacaaagtg gttggtaagc 481 ctatccctaa ccctctcctc ggtctcgatt ctacgtagta atgagctagc gctaaccggt 541 ggcgcgttaa gtcgacaatc aacctctgga tta