Construct: ORF ccsbBroad304_12885
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006588.1_s304c1, BRDN0000411530
- Derived from:
- ccsbBroadEn_12885
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- ZFYVE19 (84936)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12885
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 84936 | ZFYVE19 | zinc finger FYVE-type conta... | NM_032850.5 | 84% | 83% | (many diffs) |
| 2 | human | 84936 | ZFYVE19 | zinc finger FYVE-type conta... | NM_001077268.2 | 84% | 83.8% | 1_225del;1126T>G |
| 3 | human | 84936 | ZFYVE19 | zinc finger FYVE-type conta... | NM_001258421.2 | 74.6% | 74.4% | 0_1ins300;601T>G |
| 4 | human | 84936 | ZFYVE19 | zinc finger FYVE-type conta... | NM_001258420.2 | 69.5% | 69.4% | 1_225del;824_825ins204;922T>G |
| 5 | human | 84936 | ZFYVE19 | zinc finger FYVE-type conta... | XM_024450092.1 | 63.5% | 63.3% | 0_1ins432;469T>G |
| 6 | human | 84936 | ZFYVE19 | zinc finger FYVE-type conta... | XM_017022684.2 | 32.3% | 24.6% | (many diffs) |
| 7 | human | 84936 | ZFYVE19 | zinc finger FYVE-type conta... | XR_931925.3 | 29.2% | 1_729del;1221_1304del;1398_1399ins603 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1254
- ORF length:
- 1188
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gagtaggtgc tacggctgcg ctgtcaagtt caccctcttc aagaaggagt 121 acggctgtaa gaattgtggc agggccttct gttcaggctg cctaagcttc agtgcagcag 181 tgcctcggac tgggaacacc caacagaaag tctgcaagca atgccatgag gtcctgacca 241 gagggtcttc tgccaatgcc tccaagtggt caccacctca gaactataag aagcgtgtgg 301 cagccttgga agccaagcaa aagcccagca cttcccagag ccagggactg acacgacaag 361 accagatgat tgctgagcgc ctagcacgac tccgccagga gaacaagccc aagttagtcc 421 cctcacaggc agagatagag gcacggctgg ctgccctaaa ggatgaacgt cagggttcca 481 tcccttccac ccaggaaatg gaggcacgac ttgcagcgtt gcagggcaga gttctacctt 541 ctcaaacccc ccagccggca catcacacac cggacaccag gacccaagcc cagcagacac 601 aggatctgct aacgcagctg gcagctgagg tggctatcga tgaaagctgg aaaggaggag 661 gcccagctgc ctctctccag aatgatctca accagggtgg cccagggagc actaattcca 721 agaggcaggc caactggtcc ttggaggagg agaagagcag actgctggct gaggcagcac 781 ttgagttgcg ggaggagaac acgaggcagg aacggattct ggccctggcc aagcgactag 841 ccatgctgcg gggacaggac cccgagagag tgaccctcca ggactatcgc ctcccagaca 901 gtgatgacga cgaggatgag gagacagcca tccaaagagt cctgcagcag ctcactgaag 961 aagctgccct ggatgaggca agtggcttta acatccctgc agagcaggct tctcgaccct 1021 ggacgcaacc ccgcggggca gagcctgagg cccaggatgt ggaccccagg cctgaggctg 1081 aggaagagga gctcccctgg tgctgcatct gcaatgagga tgccacccta cgctgcgctg 1141 gctgcgatgg ggacctcttc tgtgcccgct gcttccgaga gggccatgat gcctttgagc 1201 ttaaagagca ccagacatct gcctactctc ctccacgtgc aggccaagag cactgcccaa 1261 ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta 1321 cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta