Construct: ORF ccsbBroad304_12926
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014158.1_s304c1, BRDN0000403125
- Derived from:
- ccsbBroadEn_12926
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- WDR34 (89891)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_12926
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 89891 | WDR34 | WD repeat domain 34 | NM_052844.3 | 78% | 78.1% | 1_351del;627G>A;873C>T |
| 2 | human | 89891 | WDR34 | WD repeat domain 34 | XM_011519179.2 | 72.8% | 72.9% | 1_351del;627G>A;810_811ins84 |
| 3 | mouse | 71820 | Wdr34 | WD repeat domain 34 | NM_001008498.2 | 64.8% | 67.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1323
- ORF length:
- 1257
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt catccgagag ctgaacaaga attggcagag ccacgcgttt gatggcttcg 121 aggtgaactg gaccgagcag cagcagatgg tgtcttgtct gtataccctg ggctacccgc 181 cagcccaagc gcagggtctg catgtgacca gcatctcctg gaactccact ggctctgtgg 241 tggcctgtgc ctacggccgg ctggaccatg gggactggag cacgcttaag tccttcgtgt 301 gtgcctggaa cctggaccgg cgagacctgc gtccccagca accgtcggcc gtggtggagg 361 tccccagcgc tgtcctgtgt ctggccttcc accccacgca gccctcccac gtcgcaggag 421 ggctgtacag tggtgaggtg ttggtgtggg acctgagccg tcttgaggac ccgctgctgt 481 ggcgcacagg cctgacggat gacacccaca cagaccctgt gtcccaggtg gtgtggctgc 541 ccgagcctgg gcacagccac cgcttccagg tgctgagtgt ggccactgac gggaaggtgc 601 tactctggca gggcatcggg gtaggccagc tgcagctcac agagggcttc gccctggtca 661 tgcagcagct gccacggagc accaagctca agaagcatcc ccgcggggag accgaggtgg 721 gcgccacggc agtggccttc tccagctttg accctaggct gttcattctg ggcacggaag 781 gcggcttccc gctcaagtgt tccctggcag ctggagaggc agccctcacg cggatgccca 841 gctccgtgcc cctgcgggcc ccagcacagt ttaccttctc cccccacggc ggtcccatct 901 actctgtgag ctgttccccc ttccacagga atctcttcct gagcgctggg actgacgggc 961 atgtccacct gtactccatg ctgcaggccc ctcccttgac ttcgctgcag ctctccctca 1021 agtatctgtt tgctgtgcgc tggtccccag tgcggccctt ggtttttgca gctgcctctg 1081 ggaaaggtga cgtgcagctg tttgatctcc agaaaagctc ccagaaaccc acagttttga 1141 tcaagcaaac ccaggatgaa agccctgtct actgtctgga gttcaacagc cagcagactc 1201 agctcttggc tgcgggcgat gcccagggca cagtgaaggt gtggcagctg agcacagagt 1261 tcacggaaca agggccccgg gaagctgagg acctggactg cctggcagca gaggtggcgg 1321 cctgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 1381 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1441 tctggatta