Construct: ORF ccsbBroad304_13022
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013945.1_s304c1, BRDN0000403310
- Derived from:
- ccsbBroadEn_13022
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- LOC113386 (113386)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13022
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 374928 | ZNF773 | zinc finger protein 773 | NR_130705.2 | 19% | (many diffs) | |
| 2 | human | 105372481 | ERVK3-1 | endogenous retrovirus group... | NR_144445.1 | 15.5% | 1_309del;532_1424del | |
| 3 | human | 108903150 | ZNF8-ERVK3-1 | ZNF8-ERVK3-1 readthrough | NR_144447.1 | 4.7% | 1_3588del;3811_4687del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 288
- ORF length:
- 222
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgag ccagcggaat ctgaatgcaa agacagaaca agggccgacc ggagtcacaa 121 tgacatccaa ccccataaca tggggacaga tcaagaaaac gacacaagaa gctgagaaac 181 tactggagcg ccagggtcag gcaaaaaccc ctgactccat gttcttggcc atgctagctg 241 tagtgtcctg tgcgtctata ggatcgggtg aaccaccaac cggcaattgc ccaactttct 301 tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt 361 aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta