Construct: ORF ccsbBroad304_13153

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF007615.1_s304c1, BRDN0000407742
Derived from:
ccsbBroadEn_13153
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PLEKHH2 (130271)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13153
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 130271 PLEKHH2 pleckstrin homology, MyTH4 ... XM_017003352.1 77.9% 76.2% (many diffs)
2 human 130271 PLEKHH2 pleckstrin homology, MyTH4 ... XR_001738621.1 69.4% (many diffs)
3 human 130271 PLEKHH2 pleckstrin homology, MyTH4 ... XR_001738620.1 68.8% (many diffs)
4 human 130271 PLEKHH2 pleckstrin homology, MyTH4 ... NM_172069.4 52.8% 51.7% (many diffs)
5 human 130271 PLEKHH2 pleckstrin homology, MyTH4 ... XM_017003351.2 50.1% 48.9% (many diffs)
6 human 130271 PLEKHH2 pleckstrin homology, MyTH4 ... XM_024452695.1 48.5% 47.3% (many diffs)
7 human 130271 PLEKHH2 pleckstrin homology, MyTH4 ... XR_001738622.1 34.5% (many diffs)
8 human 130271 PLEKHH2 pleckstrin homology, MyTH4 ... XR_939657.2 34.1% (many diffs)
9 human 130271 PLEKHH2 pleckstrin homology, MyTH4 ... XR_426981.2 34% (many diffs)
10 human 130271 PLEKHH2 pleckstrin homology, MyTH4 ... XM_017003353.1 15.2% 14% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
2454
ORF length:
2388
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc agagctttct gagccagagg gaccagtaga ttggaaggaa cgatgtgtag
121ctctggagtc ccaactcatg aaatttagag ttcaagcaag caagatacga gagcttttag
181cagagaagat gcaacagctt gagagacaag ttattgatgc tgaacgtcaa gcagaaaaag
241cttttcaaca ggtacaagtt atggaagata aattaaaagc agctaatatt caaaccagtg
301aatcagagac aagattatat aataagtgtc aagatctgga gtcgctaata caggaaaaag
361atgacgtcat tcaaaacttg gaattgcaac ttgaagagca gaaacaaata agaatacaag
421aagctaaaat aatagaagag aaagcagcta agataaaaga atgggtaaca gttaagttaa
481atgagctgga attggagaat cagaatcttc gtttgatcaa ccaaaaccaa actgaagaga
541taagaacaat gcagtcaaaa ctacaagaag ttcaaggaaa gaagtcatcc actgtctcta
601cactaaagct ttcggaaggc cagcgcctga gcagtttgac ctttgggtgc tttttatctc
661gagcaaggag tcctcctcaa gtagtaaaat ctgaggaaat gagcaagata tcatcgaaag
721aacctgagtt cactgaagga aaagacatgg aagaaatgga aattccagaa aagtctgttg
781ataaccaagt tctagaaaac aacagaggcc agagaacatt gcatcaaacc ccttgtggct
841cagaacagaa tcggaaaaca agaacaagct ttgccacaga tggtggcatc tcccagaatt
901ctggggctcc tgtgagtgac tggagctctg atgaggaaga cgggagcaaa ggaagatcca
961agtccagatg cacatccacc ctctccagtc acacatctga ggaaggggtc cagtgtagca
1021ggatgggaag tgaaatgtat ctgacagcat ctgatgacag cagctctata tttgaggaag
1081agacttttgg cataaagaga ccagaacaca agaagctata ttcttggcag caggaggcac
1141agtggaaagc tctaaatagt cctcttggaa agggaaattc tgaattaagt aaaaaggaac
1201aagatagttc ctcggatgaa ctgaataaaa aatttcaatc ccagagactc gattattcat
1261cttcatcgag tgaagccaac accccaagcc ctattttgac cccagcttta atgccaaagc
1321atcctaactc actctctgga aaaggaacac aattagtgcc ttcatcacac ctgccacccc
1381caaagttaag gattcctaat gttttcagta taagtgtagc actagccaaa aggcacttaa
1441gccagccaca gttaagctct gacaggatgt ttggtacaaa tagaaacgct ataagcatga
1501tacgaccact gagacctcag gaaactgatc ttgatctagt tgatggagac agtacagaag
1561ttttagagaa tatggacacg agttgtgatg atggattatt ttcctatgac tccttggact
1621ctccaaattc agatgaccag gaacactgtg actcagcaaa gaaggtggca tacagcaaac
1681ctccaactcc tcccctgcac cgttttcctt cttgggaaag cagaatttat gctgtagcca
1741aatcaggtat tcgaatgtct gaggccttca atatggagag tgttaataaa aattctgctg
1801caaccctttc ctatactaca tcaggacttt atacatctct gatatacaag aacatgacca
1861ccccagtgta tacaactttg aaggggaagg cgacccaaat aagtagcagc cctttcctgg
1921atgactcatc tgggtcagag gaagaagaca gctccagatc cagctcccgg acgtcagagt
1981cagactcacg cagtaggagt gggccaggca gccccagagc catgaaacga ggtgtgtctc
2041tctcctctgt ggcttctgaa agtgattatg ctattcctcc tgatgcttac tccacagaca
2101cggagtactc acagccagag cagaagctcc caaaaacttg ctcatcttcc agtgataatg
2161ggaaaaatga accactggaa aaatctggtt atttattaaa aatgagtggt aaagtcaagt
2221cttggaagcg gcggtggttt gttcttaaag gtggtgaatt actttactac aaatctccga
2281gtgatgtaat tagaaaaccc cagggccata ttgaacttag tgcatcctgt agtattttaa
2341gaggagataa caaacaaaca gttcaggtac ttaacttttt tttttttttt tttttttttg
2401tatcatgcca gactcaattc tcaattatcc aacctaatgg aaaggagata ggatacccaa
2461ctttcttgta caaagtggtt ggtaagccta tccctaaccc tctcctcggt ctcgattcta
2521cgtagtaatg agctagcgct aaccggtggc gcgttaagtc gacaatcaac ctctggatta

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