Construct: ORF ccsbBroad304_13226
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF015633.1_s304c1, BRDN0000411260
- Derived from:
- ccsbBroadEn_13226
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RAD9B (144715)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13226
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
---|---|---|---|---|---|---|---|---|
1 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | NM_001286533.2 | 95.2% | 94% | (many diffs) |
2 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | NM_001286534.2 | 95.2% | 94% | (many diffs) |
3 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | XM_017018882.2 | 95.2% | 94% | (many diffs) |
4 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | NM_001368054.1 | 80.8% | 79.7% | (many diffs) |
5 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | NM_001368055.1 | 80.8% | 79.7% | (many diffs) |
6 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | XM_017018879.2 | 80.8% | 79.7% | (many diffs) |
7 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | XM_017018880.2 | 80.8% | 79.7% | (many diffs) |
8 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | NM_001286531.2 | 72.2% | 71.4% | (many diffs) |
9 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | NM_001286532.2 | 72.2% | 71.4% | (many diffs) |
10 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | NM_001368051.1 | 72.2% | 71.4% | (many diffs) |
11 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | XM_024448860.1 | 72.2% | 71.4% | (many diffs) |
12 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | XM_024448861.1 | 72.2% | 71.4% | (many diffs) |
13 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | XM_005253849.5 | 68.8% | 62.7% | (many diffs) |
14 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | XM_017018877.2 | 68.4% | 67.6% | (many diffs) |
15 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | NM_001368056.1 | 67.8% | 66.1% | 0_1ins117;533_534ins109;588_639del |
16 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | XM_017018881.2 | 67.8% | 66.1% | 0_1ins117;533_534ins109;588_639del |
17 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | XM_011537972.3 | 63.7% | 62.9% | (many diffs) |
18 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | NM_152442.4 | 63.1% | 63.1% | 1_474del |
19 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | NM_001368053.1 | 62.6% | 61.2% | 1_258del;908_909ins109;963_1014del |
20 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | NM_001286535.2 | 60.1% | 59.4% | (many diffs) |
21 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | NM_001286536.2 | 59.5% | 52.8% | (many diffs) |
22 | human | 144715 | RAD9B | RAD9 checkpoint clamp compo... | NM_001368052.1 | 52.5% | 51.1% | 1_474del;1124_1125ins109;1179_1230del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 879
- ORF length:
- 813
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgat tcaaccaaga ttgcttgctg atgccattgt tctttttaca tcaagtcaag 121 aggaagttac tcttgctgtt actccactga atttttgcct caagagttct aatgaggaat 181 caatggattt gagcaatgct gtacacagtg agatgtttgt tggctcagat gagtttgact 241 tctttcaaat tggaatggac actgagataa cattttgttt caaagaattg aagggaatac 301 tgacattttc agaagctaca catgctccta tatccattta ttttgatttc cctgggaaac 361 ctctggcttt gagtattgat gatatgttag tggaagctaa ctttattttg gccacattag 421 ctgatgaaca aagtagagca tcttcaccac agtcactgtg tctttcacag aaacgaaaaa 481 ggtcagatct gattgaaaaa aaggctggca aaaatgtaac tggccaggcc ctggaatgta 541 tttcaaaaaa agcagcacca agaaggcttt atcctaagga gactctcaca aacatatctg 601 cattggaaaa ctgtggcagc cctgcaatga aaagagtgga tggagatgtc agtgaagtat 661 cagaaagcag tgtcagcaac acagaggaag tgccagggtc tctgtgtctc agaaagtttt 721 cttgcatgtt ctttggagca gtttcttctg accagcaaga acacttcaac caccctttcg 781 acagtctggc aagagcaagt gacagtgaag aggacatgaa taatgtgtgc tgcaggaaag 841 aatttaatgg aagtgatgcc aaatatttct gtattatctg cccaactttc ttgtacaaag 901 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 961 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta