Construct: ORF ccsbBroad304_13289
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003445.1_s304c1, BRDN0000403474
- Derived from:
- ccsbBroadEn_13289
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TRMT44 (152992)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13289
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 152992 | TRMT44 | tRNA methyltransferase 44 h... | NM_001350233.1 | 79.3% | 78.1% | (many diffs) |
| 2 | human | 152992 | TRMT44 | tRNA methyltransferase 44 h... | XM_011513409.3 | 79.3% | 78.1% | (many diffs) |
| 3 | human | 152992 | TRMT44 | tRNA methyltransferase 44 h... | XM_011513408.1 | 77.5% | 74.4% | (many diffs) |
| 4 | human | 152992 | TRMT44 | tRNA methyltransferase 44 h... | XM_011513407.2 | 57.4% | 55.2% | (many diffs) |
| 5 | human | 152992 | TRMT44 | tRNA methyltransferase 44 h... | XM_011513406.2 | 54.2% | 52.8% | (many diffs) |
| 6 | human | 152992 | TRMT44 | tRNA methyltransferase 44 h... | NM_152544.3 | 54.1% | 52% | (many diffs) |
| 7 | human | 152992 | TRMT44 | tRNA methyltransferase 44 h... | XR_924916.2 | 34.4% | (many diffs) | |
| 8 | human | 152992 | TRMT44 | tRNA methyltransferase 44 h... | XR_427468.3 | 30.4% | (many diffs) | |
| 9 | human | 152992 | TRMT44 | tRNA methyltransferase 44 h... | XR_001741150.2 | 29.5% | (many diffs) | |
| 10 | human | 152992 | TRMT44 | tRNA methyltransferase 44 h... | XM_011513410.2 | 29.1% | 25.6% | (many diffs) |
| 11 | human | 152992 | TRMT44 | tRNA methyltransferase 44 h... | XM_017007811.1 | 28% | 24.1% | (many diffs) |
| 12 | human | 152992 | TRMT44 | tRNA methyltransferase 44 h... | XR_001741149.1 | 27.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1299
- ORF length:
- 1233
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtg tatcaaattc agctcagtca tagcaaagaa gaatgaaaga tggcattcag 121 atggaatcgt gtatcccaaa cccacgtggc ttggagaaga gttgctggcc aagttggcca 181 agtggtctgt agagaacaag aagagtgact ttaaaagcac cctttccctc atctccatta 241 tgaagtatag caaggcttac caggaactta aagagaagta taaggaaatg gttaaggtgt 301 ggcctgaagt cactgatcct gagaagttcg tgtatgaaga tgtggctatc gcagcatacc 361 tgctgattct atgggaagaa gaaagggctg agaggagact aactgccagg cagtcctttg 421 tggacctggg atgtggaaat ggcctcctgg tccacatcct gagcagtgag gggcatccag 481 gcagagggat tgatgtccga agaagaaaaa tctgggacat gtatggacca caaactcagt 541 tagaggaaga tgcaatcaca cccaatgata agaccctttt ccctgatgtt gattggttaa 601 tcggtaacca ttctgatgaa ctcacaccat ggatacctgt cattgcagcc aggtcttcct 661 acaattgccg cttctttgtc ctcccctgct gcttctttga cttcattgga agatactccc 721 ggaggcagag taagaagact cagtaccggg aataccttga cttcattaaa gaagtgggct 781 tcacctgtgg gtttcacgtg gacgaagact gcctcaggat tccttcaacc aaaagagtct 841 gtctcgttgg aaaatccaga acataccctt cctccagaga agcttccgtg gatgaaaaga 901 ggactcagta cattaagagc aggcggggct gccctgtaag cccacctggc tgggagcttt 961 ccccttctcc acgctgggtt gctgctggca gtgctggtca ctgtgacggt cagcaagctc 1021 tggacgccag ggtcgggtgt gtaaccaggg cctgggccgc tgagcatgga gcagggcccc 1081 aggctgaagg accctggcta cctggatttc atcccagaga aaaggctgag cgtgtgagga 1141 actgtgccgc cctgccacga gattttattg accaagtggt tttgcaagta gcgaatttac 1201 tgttaggtgg aaagcaatta aacacaagaa gttctcgaaa tgggagtttg aagacctgga 1261 atgggggagg taagctgtcc accatcttag ggaggggcta cccaactttc ttgtacaaag 1321 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 1381 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta