Construct: ORF ccsbBroad304_13391
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010395.1_s304c1, BRDN0000402855
- Derived from:
- ccsbBroadEn_13391
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STT3B (201595)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13391
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 201595 | STT3B | STT3 oligosaccharyltransfer... | XM_017005858.1 | 33% | 32.9% | 1_1365del;2027C>T |
| 2 | human | 201595 | STT3B | STT3 oligosaccharyltransfer... | XM_017005859.2 | 29.7% | 29.2% | (many diffs) |
| 3 | human | 201595 | STT3B | STT3 oligosaccharyltransfer... | NM_178862.3 | 27.1% | 27.1% | 1_1803del;2465C>T |
| 4 | human | 201595 | STT3B | STT3 oligosaccharyltransfer... | XM_017005857.2 | 24.4% | 24% | (many diffs) |
| 5 | mouse | 68292 | Stt3b | STT3, subunit of the oligos... | NM_024222.2 | 24.4% | 26.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 741
- ORF length:
- 675
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtc ttggtgggat tatggctatc agatagctgg aatggctaat agaactacgt 121 tggtggataa taacacctgg aataacagcc acatagcact ggtgggaaaa gctatgtctt 181 ctaatgaaac agcagcctat aaaatcatga ggactctaga tgtagattat gttttggtta 241 tttttggagg ggttattggc tattctggtg atgatatcaa caaatttctc tggatggtta 301 ggatagctga aggagaacat cccaaagaca ttcgggaaag tgactatttt accccacagg 361 gagaattccg tgtagacaaa gcaggatccc ctactttgtt gaattgcctt atgtataaaa 421 tgtcatacta cagatttgga gaaatgcagc tggattttcg tacaccccca ggttttgacc 481 gaacacgtaa tgctgagatt ggaaataagg acattaaatt caaacatttg gaagaagcct 541 ttacatcaga acactggctt gttaggatat ataaagtaaa agcacctgat aacagggaga 601 cattagatca caaacctcga gtcaccaaca ttttcccaaa acagaagtat ttgtcaaaga 661 agactaccaa aaggaagcgt ggctacatta aaaataagct ggtttttaag aaaggcaaga 721 aaatatttaa gaagactgtt tacccaactt tcttgtacaa agtggttggt aagcctatcc 781 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 841 ttaagtcgac aatcaacctc tggatta