Construct: ORF ccsbBroad304_13428
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014627.1_s304c1, BRDN0000411473
- Derived from:
- ccsbBroadEn_13428
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- FGD2 (221472)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13428
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XM_011514373.2 | 25.7% | 25.3% | (many diffs) |
| 2 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XM_011514372.2 | 25.6% | 25.1% | (many diffs) |
| 3 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XR_926106.1 | 22.4% | (many diffs) | |
| 4 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XR_001743248.1 | 16.7% | (many diffs) | |
| 5 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | NM_173558.4 | 15.6% | 15.4% | (many diffs) |
| 6 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XM_017010429.2 | 15.5% | 15.2% | (many diffs) |
| 7 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XR_001743243.1 | 9.4% | (many diffs) | |
| 8 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XR_001743242.2 | 9.3% | (many diffs) | |
| 9 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XR_001743244.2 | 7.4% | (many diffs) | |
| 10 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XR_001743241.2 | 7.1% | (many diffs) | |
| 11 | human | 221472 | FGD2 | FYVE, RhoGEF and PH domain ... | XR_001743240.2 | 7.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 378
- ORF length:
- 312
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa gggggcaagt gaggagaagc tggcatctgt gtccaacctg gtcactgtgt 121 ttgagaatag caggacccca gaagcagcac ccagaggcca caggctagag gacgtgcatc 181 accgccctga gtgcaggcct cccgagtccc caggaccacg ggagaagacg aatgtcgggg 241 aggccgtggg gtctgagccc aggacagtca gcaggaggta cctgaactcc ctgaagaaca 301 agctgtccag cgaagcctgg aggaaatctt gccagcctgt gaccctctca ggatcgggga 361 cgcaggtgcc tgagggctgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 421 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 481 agtcgacaat caacctctgg atta