Construct: ORF ccsbBroad304_13470
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF017415.1_s304c1, BRDN0000400735
- Derived from:
- ccsbBroadEn_13470
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RASGEF1C (255426)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13470
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 255426 | RASGEF1C | RasGEF domain family member 1C | NM_175062.4 | 47% | 45.8% | 1_453del;1080_1081ins38;1130_1398del |
| 2 | mouse | 74563 | Rasgef1c | RasGEF domain family, membe... | XM_006534378.3 | 43.7% | 45.7% | (many diffs) |
| 3 | mouse | 74563 | Rasgef1c | RasGEF domain family, membe... | NM_029004.1 | 41.8% | 43.6% | (many diffs) |
| 4 | mouse | 74563 | Rasgef1c | RasGEF domain family, membe... | XM_030246404.1 | 41.8% | 43.6% | (many diffs) |
| 5 | mouse | 74563 | Rasgef1c | RasGEF domain family, membe... | XM_030246405.1 | 41.8% | 43.6% | (many diffs) |
| 6 | mouse | 74563 | Rasgef1c | RasGEF domain family, membe... | NM_001347462.1 | 41.5% | 43.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 780
- ORF length:
- 714
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca tcagctccta caggctctgc accagaagct ggcggctctg cgccaggggc 121 cagaaggtct ggtgggtgcc gacaagccca tctcctacag gaccaagcca ccagcctcca 181 tccacaggga gctccttggt gtctgcagcg acccctacac actggcccag cagctgaccc 241 acgtggaact ggagcggctg cggcacatcg ggcctgagga gtttgtccag gcctttgtga 301 acaaggaccc tctggccagc acaaagccct gcttcagtga caagaccagc aacctggagg 361 cttatgtgaa atggttcaac aggctgtgct acctggtggc aactgagatc tgcatgccag 421 ccaagaagaa gcagagggcc caggtgattg agttcttcat cgacgtggcc cgcgagtgct 481 tcaacatcgg caacttcaac tccctcatgg ccatcatctc cggcatgaac atgagccctg 541 tctccaggct gaagaagacc tgggccaaag tgaggacggc caagtttttc atcctcgagc 601 accagatgga cccaacgggg aatttctgca actacaggac agccctgcgc ggggcggccc 661 accgctccct gacggcccac agcagccgag agaaggaggc caccttggga ggccgaggcg 721 agcagatcac aagattgtca ttcctttctt cagcctgctc atcaaagaca tctacttcct 781 gcccaacttt cttgtacaaa gtggttggta agcctatccc taaccctctc ctcggtctcg 841 attctacgta gtaatgagct agcgctaacc ggtggcgcgt taagtcgaca atcaacctct 901 ggatta