Construct: ORF ccsbBroad304_13473

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF003622.1_s304c1, BRDN0000461930
Derived from:
ccsbBroadEn_13473
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
PAN3 (255967)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13473
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 255967 PAN3 poly(A) specific ribonuclea... XM_017020533.1 99.9% 100% 273T>C
2 human 255967 PAN3 poly(A) specific ribonuclea... XM_005266334.1 96.1% 96.1% 1_66del;339T>C
3 human 255967 PAN3 poly(A) specific ribonuclea... XM_017020532.1 96.1% 96.1% 1_66del;339T>C
4 human 255967 PAN3 poly(A) specific ribonuclea... XM_017020531.1 85% 85% 1_291del;564T>C
5 human 255967 PAN3 poly(A) specific ribonuclea... XM_017020529.2 83.6% 83.6% 1_324del;597T>C
6 human 255967 PAN3 poly(A) specific ribonuclea... XM_011535034.3 74.5% 74.6% 1_564del;837T>C
7 human 255967 PAN3 poly(A) specific ribonuclea... XM_005266333.3 66.3% 66.3% 1_840del;1113T>C
8 human 255967 PAN3 poly(A) specific ribonuclea... NM_175854.8 62.3% 62.3% 1_1002del;1275T>C
9 human 255967 PAN3 poly(A) specific ribonuclea... XM_005266332.3 62.1% 62.2% 1_1002del;1275T>C;1408_1409insCAG
10 human 255967 PAN3 poly(A) specific ribonuclea... XM_011535032.3 51.8% 51.8% 1_1002del;1275T>C;2385_2385delCins277
11 human 255967 PAN3 poly(A) specific ribonuclea... XM_011535033.2 45.8% 44.2% (many diffs)
12 human 255967 PAN3 poly(A) specific ribonuclea... XM_017020528.1 40.1% 39.5% (many diffs)
13 human 255967 PAN3 poly(A) specific ribonuclea... XR_941549.2 38.4% (many diffs)
14 human 255967 PAN3 poly(A) specific ribonuclea... XM_017020530.2 36.1% 35.8% (many diffs)
15 mouse 72587 Pan3 PAN3 poly(A) specific ribon... XM_006504878.3 91.6% 97.1% (many diffs)
16 mouse 72587 Pan3 PAN3 poly(A) specific ribon... XM_006504877.3 91.1% 96.6% (many diffs)
17 mouse 72587 Pan3 PAN3 poly(A) specific ribon... XM_006504874.2 88.1% 93.4% (many diffs)
18 mouse 72587 Pan3 PAN3 poly(A) specific ribon... XM_006504873.3 69.3% 64.3% (many diffs)
19 mouse 72587 Pan3 PAN3 poly(A) specific ribon... NM_028291.4 60.9% 64.6% (many diffs)
20 mouse 72587 Pan3 PAN3 poly(A) specific ribon... XM_006504871.3 58.8% 62.3% (many diffs)
21 mouse 72587 Pan3 PAN3 poly(A) specific ribon... XM_006504870.3 57.6% 61.2% (many diffs)
22 mouse 72587 Pan3 PAN3 poly(A) specific ribon... XM_006504868.3 57.2% 60.7% (many diffs)
23 mouse 72587 Pan3 PAN3 poly(A) specific ribon... XM_006504869.3 57.1% 60.6% (many diffs)
24 mouse 72587 Pan3 PAN3 poly(A) specific ribon... XM_006504872.3 53.9% 56.4% (many diffs)
25 mouse 72587 Pan3 PAN3 poly(A) specific ribon... XM_017321113.1 53.2% 56.2% (many diffs)
26 mouse 72587 Pan3 PAN3 poly(A) specific ribon... XM_011241014.2 40.9% 43.4% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
1728
ORF length:
1659
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gtcgttgtct gctgggtctt cccctcttca ttcccccaag attactccac
121atacttctcc tgctcccaga agaagaagtc acactccaaa tccagcaagt tacatggtgc
181cttctagtgc ctctacatct gttaataatc ctgtttctca gactccgtct tctggtcagg
241tgatccaaaa ggaaactgtt ggtgggacga cttacttcta tacagacaca actccagcac
301ctttgactgg aatggtgttt ccaaactatc atatttatcc cccaactgca cctcacgttg
361cttatatgca accgaaagca aacgcacctt ccttcttcat ggctgatgaa ctccgacagg
421agctgatcaa cagacattta ataacaatgg ctcaaattga tcaagcagat atgccagcag
481ttcctacaga ggttgacagc taccatagcc tattccctct agaaccactg ccacctccca
541accggataca gaaatcaagt aattttggat atattacatc ttgctacaaa gctgtaaaca
601gcaaagatga tctgccatat tgccttcgga ggatacatgg ttttcgtctt gttaacacaa
661agtgcatggt gttggtcgac atgtggaaga aaattcaaca ctcaaatatc gtaactttgc
721gtgaagtatt taccactaaa gcatttgctg agccctctct tgtgtttgca tatgatttcc
781atgctggagg agaaactatg atgagcagac actttaatga ccctaatgct gatgcctact
841tcaccaagag aaagtggggt cagcacgagg gaccattgcc caggcagcat gctggattat
901tgccagaatc tcttatttgg gcatatattg tccaactaag ttctgcattg cgtaccattc
961atacagcagg tttggcatgt cgagttatgg atccaacaaa gattctgata actggcaaaa
1021caaggttgcg agtaaattgt gttggagttt ttgatgtttt aacatttgat aacagtcaaa
1081ataataatcc attggcatta atggcccagt accagcaagc agatctgata tcattaggaa
1141aagttgtgtt ggctttggct tgcaactctt tggcaggaat tcagcgagag aatttacaga
1201aagccatgga actggtgaca atcaactatt cctctgacct gaagaatctg attttgtatt
1261tgttgactga ccaaaacagg atgcgaagtg taaatgacat catgcccatg attggtgctc
1321gattttatac tcaattggat gctgctcaaa tgagaaatga tgtcatagag gaagaccttg
1381caaaggaggt tcaaaatgga agactgttta ggctcctagc aaaattggga acaatcaatg
1441agaggccgga gtttcagaag gatcccactt ggtcagagac tggagaccgt tatctgttga
1501aactctttag ggatcatctt tttcatcagg tgacagaagc aggtgctccc tggattgacc
1561tcagtcatat aatttcttgt cttaacaagc tagatgctgg tgtgccagaa aaaataagcc
1621tgatttccag agatgagaag agtgtacttg tggtgaccta cagtgactta aagcgctgct
1681ttgaaaatac ttttcaagaa ctgattgcag ctgcaaatgg tcagttgttg ccaactttct
1741tgtacaaagt ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt
1801aatgagctag cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

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