Construct: ORF ccsbBroad304_13477

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF004636.1_s304c1, BRDN0000410013
Derived from:
ccsbBroadEn_13477
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
SUN3 (256979)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13477
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 256979 SUN3 Sad1 and UNC84 domain conta... NM_001284350.2 88.3% 84.8% 1_22del;49_146del;343A>G
2 human 256979 SUN3 Sad1 and UNC84 domain conta... NM_001030019.2 85.3% 85.1% 1_156del;379A>G
3 human 256979 SUN3 Sad1 and UNC84 domain conta... NM_152782.4 85.3% 85.1% 1_156del;379A>G
4 human 256979 SUN3 Sad1 and UNC84 domain conta... XM_017011930.1 85.3% 85.1% 1_156del;379A>G
5 human 256979 SUN3 Sad1 and UNC84 domain conta... XR_002956417.1 59.2% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
981
ORF length:
915
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgct tacactgact tttcttcttg taggactcct aaatcatcag tggcttaaag
121aaacagatgt tcctcagaaa tccagacaat tatatgccat aattgcagaa tatggttcaa
181ggctttataa atatcaggcc agacttcgta tgcctaaaga gcaactggaa cttttaaaga
241aggaaagcca gaatctggaa aacaattttc gtcaaattct atttttggtc gaacaaatag
301atgtcctgaa ggcattgcta agagatatga aggatggtat ggacaataat cacaactgga
361acacccatgg agaccctgtg gaggacccgg accacacaga ggaagtgtca aacttggtca
421attatgtact taaaaagttg agagaagacc aagtcgagat ggctgattat gccctgaagt
481cggccggagc ctccatcatt gaagctggga cctcagaaag ttataaaaat aataaagcaa
541aattgtactg gcatgggata ggtttcctaa atcatgaaat gcctccagat attattcttc
601agccggatgt ctaccctgga aagtgctggg cttttccagg ttcccagggt cataccctaa
661tcaagcttgc tacaaagatc ataccaactg ctgttaccat ggagcacatc tcagagaagg
721tgtctccgtc aggaaacatc tccagtgcac ccaaggaatt ttctgtctat ggcatcacaa
781aaaaatgtga aggagaagaa attttcctag gtcagtttat atataacaaa acaggaacca
841ccgttcaaac atttgaactc cagcatgcag tttctgaata tttattatgt gtgaaactta
901atatctttag caactgggga cacccgaagt atacttgttt atatcgattc agggtccatg
961gcacaccagg caagcacatc tacccaactt tcttgtacaa agtggttggt aagcctatcc
1021ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg
1081ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)