Construct: ORF ccsbBroad304_13542
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF006737.1_s304c1, BRDN0000412136
- Derived from:
- ccsbBroadEn_13542
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- RUFY4 (285180)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13542
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285180 | RUFY4 | RUN and FYVE domain contain... | NM_198483.3 | 69.7% | 69.7% | 1_519del |
| 2 | human | 285180 | RUFY4 | RUN and FYVE domain contain... | XM_011511014.2 | 69.7% | 69.7% | 1_519del |
| 3 | human | 285180 | RUFY4 | RUN and FYVE domain contain... | XM_017003896.1 | 69.7% | 69.7% | 1_519del |
| 4 | human | 285180 | RUFY4 | RUN and FYVE domain contain... | XM_017003895.1 | 69.5% | 69.5% | 1_522del |
| 5 | human | 285180 | RUFY4 | RUN and FYVE domain contain... | XM_017003897.1 | 63.7% | 63.6% | 1_522del;1617_1617delCins100 |
| 6 | human | 285180 | RUFY4 | RUN and FYVE domain contain... | NR_034176.1 | 32.8% | 1_1898del;3093_3638del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 1263
- ORF length:
- 1194
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gttctcagag tcacgctgct ccagttccac ccaaacccag ggaaggagac 121 ccagaaaaaa caaagatgcc ccaaagaaga tcccagccgc atatggaggg cctgaaaatg 181 tccagattga ggactcacac accagtcaag ccatctgtct gcaagatgca cccagtggac 241 agcagctggc agggcttccc aggtcccagc aacaaaggca tcttcctttc tttttggaaa 301 agaaggggga aagttccagg aaacataggt acccccagag catgtgggag ccagaaggga 361 aggagcttca gctagaccag gaggaaagag ccccatggat tgagatcttc ctggggaact 421 caacacccag cacccaggga caggggaagg gggctatggg cactcagaag gaggtgatag 481 ggatggaggc tgaggtcaca ggggttctgc tggttgcaga gggtcagaga acaacagagg 541 ggactcacaa aaaggaagca gagtggagtc acgtccagag gctgctgatg cccagcccca 601 gaggggctgt agagggagca gtatcaggga gcaggcaggg gtcggggggc tctagcatcc 661 tgggggagcc ctgggtcctt cagggacacg caacaaagga agactctacc gtggagaatc 721 cacaagtgca aacagaagtg acccttgtgg ccagaaggga ggagcaagcc gaggtgtccc 781 tgcaggacga gatcaagagc ctcagacttg ggctccggaa ggctgaggag caggcccagc 841 gccaggagca gctgctgagg gagcaggagg gggagctgca ggcacttcgg gagcagctca 901 gcaggtgtca ggaagagaga gccgagctgc aggcacagct ggagcagaag caacaggagg 961 ctgagaggag ggatgccatg taccaggagg agcttggagg gcagcgggac ttggtccagg 1021 ccatgaagag gcgggtgttg gaactgatcc aagagaagga ccgcctgtgg cagaggctcc 1081 agcatctctc ttccatggct cccgagtgct gtgtggcctg tagcaagatc tttggccgat 1141 tttctcggcg gtatccatgc aggctctgtg gaggcctgct ctgccatgct tgctccatgg 1201 attacaagaa gagagaccgc tgctgcccac cctgcgccca gggaagagaa gcccaggtca 1261 ccttgccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc 1321 tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc 1381 tctggatta