Construct: ORF ccsbBroad304_13556
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF011949.1_s304c1, BRDN0000460225
- Derived from:
- ccsbBroadEn_13556
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SREK1IP1 (285672)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13556
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 285672 | SREK1IP1 | SREK1 interacting protein 1 | NM_173829.4 | 64.1% | 52.2% | 235_236insA;300_465del |
| 2 | human | 285672 | SREK1IP1 | SREK1 interacting protein 1 | XM_011543344.3 | 53.8% | 41.5% | 13_14ins48;187_188insA;252_417del |
| 3 | mouse | 67288 | Srek1ip1 | splicing regulatory glutami... | NM_026075.3 | 55.9% | 50.9% | (many diffs) |
| 4 | mouse | 67288 | Srek1ip1 | splicing regulatory glutami... | NR_135272.1 | 13.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 369
- ORF length:
- 300
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcagtccca ggttgcaaca aggacagtgt cagagcaggc tgtaaaaaat 121 gtggctaccc tggtcacctg acttttgaat gccgcaattt tctccgagta gaccctaaaa 181 gggacatagt tttggatgtc agcagtacaa gtagtgaaga tagcgatgaa gagaatgaag 241 aactgaataa attgcaggca ttacaggaaa aaagaataaa tgaagaagag gaaaagaaga 301 aagaaaaaaa gcaaagaaaa aatcaaattg aaaaaaaaaa ggaaaaggtc ttactcatcc 361 agttccactt gccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 421 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 481 tcaacctctg gatta