Transcript: Mouse NM_026075.3

Mus musculus splicing regulatory glutamine/lysine-rich protein 1interacting protein 1 (Srek1ip1), transcript variant 1, mRNA.

Source:
NCBI, updated 2017-06-10
Taxon:
Mus musculus (mouse)
Gene:
Srek1ip1 (67288)
Length:
1789
CDS:
185..646

Additional Resources:

NCBI RefSeq record:
NM_026075.3
NBCI Gene record:
Srek1ip1 (67288)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Mouse NM_026075.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Mouse Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000247236 GGTAGATCCTGGGTTGATTTA pLKO_005 1344 3UTR 100% 13.200 10.560 N Srek1ip1 n/a
2 TRCN0000247237 TAACAGCGTCTTGGGCCTAAC pLKO_005 644 CDS 100% 6.000 4.800 N Srek1ip1 n/a
3 TRCN0000257801 AGTAGTGAGGACAGTGATGAA pLKO_005 326 CDS 100% 4.950 3.465 N Srek1ip1 n/a
4 TRCN0000247235 TCTTACTCCAGTGCCACTGAA pLKO_005 464 CDS 100% 4.950 3.465 N Srek1ip1 n/a
5 TRCN0000190118 CCTTACCATTCTGAACTCACC pLKO.1 617 CDS 100% 2.160 1.512 N Srek1ip1 n/a
6 TRCN0000202145 CTACCCTGGTCATCTGACTTT pLKO.1 241 CDS 100% 4.950 2.970 N Srek1ip1 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_026075.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_05406 pDONR223 100% 85.8% 89.6% None (many diffs) n/a
2 ccsbBroad304_05406 pLX_304 0% 85.8% 89.6% V5 (many diffs) n/a
3 TRCN0000479158 AGTCATGGAACATCCTTTATACGT pLX_317 58.7% 85.5% 77.7% V5 (not translated due to frame shift) (many diffs) n/a
4 ccsbBroadEn_13556 pDONR223 100% 55.9% 50.9% None (many diffs) n/a
5 ccsbBroad304_13556 pLX_304 0% 55.9% 50.9% V5 (many diffs) n/a
6 TRCN0000476011 CCGCTTAAACATTCTTTATGGCCC pLX_317 100% 55.9% 50.9% V5 (many diffs) n/a
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