Construct: ORF ccsbBroad304_13573
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008709.1_s304c1, BRDN0000404218
- Derived from:
- ccsbBroadEn_13573
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DHRS4L2 (317749)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13573
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 317749 | DHRS4L2 | dehydrogenase/reductase 4 l... | NM_198083.4 | 98.9% | 98.7% | 1_6delATGCAG;55A>T |
| 2 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | NM_021004.4 | 80.4% | 70% | (many diffs) |
| 3 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | XM_006720005.3 | 79.6% | 69% | (many diffs) |
| 4 | human | 317749 | DHRS4L2 | dehydrogenase/reductase 4 l... | NM_001193635.1 | 72.7% | 67.9% | (many diffs) |
| 5 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | NM_001282987.2 | 71.6% | 58.6% | (many diffs) |
| 6 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | NM_001282988.2 | 68.4% | 58.4% | (many diffs) |
| 7 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | NM_001282989.2 | 67.3% | 57.2% | (many diffs) |
| 8 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | NM_001282990.2 | 66.2% | 55.9% | (many diffs) |
| 9 | human | 728635 | DHRS4L1 | dehydrogenase/reductase 4 l... | XM_006720246.3 | 65.3% | 52.8% | (many diffs) |
| 10 | human | 317749 | DHRS4L2 | dehydrogenase/reductase 4 l... | NM_001193636.1 | 56.9% | 56.9% | 0_1ins297 |
| 11 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | XM_011536366.2 | 56.1% | 53% | (many diffs) |
| 12 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | NM_001282991.2 | 55% | 44.2% | (many diffs) |
| 13 | human | 317749 | DHRS4L2 | dehydrogenase/reductase 4 l... | NM_001193637.1 | 39.1% | 39.1% | 0_1ins297;105_106ins123 |
| 14 | human | 728635 | DHRS4L1 | dehydrogenase/reductase 4 l... | NM_001277864.2 | 33.7% | 19.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 756
- ORF length:
- 690
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc caggctgcta ggcctctgtg cctgggcacg gaagtcggtg cggttggcca 121 gctccaggat gacccgccgg gacccgctca caaataaggt ggccctggta acggcctcca 181 ccgacgggat cggcttcgcc atcgcccggc gtttggccca ggacagggcc cacgtggtcg 241 tcagcagccg gaagcagcag aatgtggacc aggcggtggc cacgctgcag ggggaggggc 301 tgagcgtgac gggcactgtg tgccatgtgg ggaaggcgga ggaccgggag cggctggtgg 361 ccatggctgt gaagcttcat ggaggtatcg atatcctagt ctccaatgct gctgtcaacc 421 ctttctttgg aagcctaatg gatgtcaccg aggaggtgtg ggacaagact ctggacatta 481 atgtgaaggc cccagccctg atgacaaagg cagtggtgcc agaaatggag aaacgaggag 541 gcggctcagt ggtgatcgtg tcttccatag cagccttcag tccatctcct ggcttcagtc 601 cttacaatgt cagtaaaaca gccttgctgg gcctcaacaa taccctggcc atagagctgg 661 ccccaaggaa cattagggtg aactgcctgc acctggactt atcaagacta gcttcagcag 721 gatgctctgg atggacaagg aaaaagagga aagcatgccc aactttcttg tacaaagtgg 781 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 841 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta