Construct: ORF ccsbBroad304_13584

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF018039.1_s304c1, BRDN0000402899
Derived from:
ccsbBroadEn_13584
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
METTL2A (339175)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13584
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 339175 METTL2A methyltransferase like 2A NM_181725.3 63.8% 63.7% 1_408del;435A>G;464T>C
2 human 55798 METTL2B methyltransferase like 2B NM_018396.2 63.3% 63.4% (many diffs)
3 human 339175 METTL2A methyltransferase like 2A XM_017024572.2 38.1% 35.1% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
792
ORF length:
726
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga agaacagcac aagtgttctt cgaagagcct tgaacataaa acacagacac
121ctcctgtgga ggagaatgta actcagaaaa ttagtgacct ggaaatttgt gctgatgagt
181ttcctggatc ctcagccacc taccgaatac tggaggttgg ctgtggtgtg ggaaacacag
241tctttccaat tttacaaacg aacaatgacc caggactctt tgtttattgc tgtgattttt
301cttccacagc tatagaactg gtccagacaa attcagaata tgatccttct cggtgttttg
361cctttgttca cgacctgtgt gatgaagaga agagttaccc agtgcccaag ggcagtcttg
421atattatcat tctcatattt gttctttcag caattgttcc agacaagatg cagaaggcta
481tcaacaggct gagcaggctt ctgaaacctg gcgggatgat gcttctgcga gattacggcc
541gctatgacat ggctcagctt cggtttaaaa aaggtcagtg tctatctgga aatttctacg
601tgagaggtga tggaaccaga gtttacttct tcacacaaga ggaactggac acgcttttca
661ccactgctgg actggaaaaa gttcagaacc tggtggatcg ccgactgcag gtgaaccgag
721gaaagcaact gacaatgtac cgggtttgga ttcagtgcaa atactgcaag ccccttctgt
781ccagcaccag ctgcccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
841tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
901caatcaacct ctggatta

Download FASTA (ORF) (Full)

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