Construct: ORF ccsbBroad304_13605
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007755.1_s304c1, BRDN0000408489
- Derived from:
- ccsbBroadEn_13605
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- STAC2 (342667)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13605
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 342667 | STAC2 | SH3 and cysteine rich domain 2 | NM_001351360.2 | 100% | 100% | |
| 2 | human | 342667 | STAC2 | SH3 and cysteine rich domain 2 | NM_198993.5 | 65.4% | 65.4% | 1_426del |
| 3 | human | 342667 | STAC2 | SH3 and cysteine rich domain 2 | XM_017024580.1 | 64.6% | 64.4% | 1_426del;942_956del |
| 4 | human | 342667 | STAC2 | SH3 and cysteine rich domain 2 | XM_017024581.1 | 53.2% | 53.2% | 1_426del;841_842ins150 |
| 5 | human | 342667 | STAC2 | SH3 and cysteine rich domain 2 | XM_017024583.1 | 34.3% | 34% | (many diffs) |
| 6 | human | 342667 | STAC2 | SH3 and cysteine rich domain 2 | XR_002957997.1 | 23% | 1_711del;1226_1227ins52;1467_3225del | |
| 7 | mouse | 217154 | Stac2 | SH3 and cysteine rich domain 2 | NM_146028.4 | 58.2% | 61% | (many diffs) |
| 8 | mouse | 217154 | Stac2 | SH3 and cysteine rich domain 2 | XR_001779960.1 | 21% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 876
- ORF length:
- 807
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gtgcaaagtc agcgtccacc tctggtgctc tgaggagatc tcccaccagc 121 aatgcccagg caagacgtcc acctccttcc gccgcaactt cagttcccct ctcctggtgc 181 atgagccgcc accagtctgt gccacaagca aagagtcccc acccactggg gacagtggga 241 aggtggaccc tgtctacgag accctgcgct atggcacctc cctggcactg atgaaccgct 301 ccagtttcag cagcacctct gagtccccga caaggagcct gagtgagcgg gatgagctga 361 ccgaggatgg ggaaggcagc atccgcagct ctgaggaggg gcctggtgac agtgcatctc 421 cagtattcac agccccagca gagagtgaag ggccaggacc agaggagaag agtcctggac 481 agcagctccc caaagccacc ctgcggaagg atgtggggcc catgtactcc tacgttgcac 541 tctacaagtt tctgccccag gagaacaatg atctggctct gcagcctgga gatcggatca 601 tgctggtgga tgactctaac gaggactggt ggaagggcaa gatcggcgac cgggttggct 661 tcttcccagc taattttgtg caacgggtga ggccaggcga gaatgtttgg cgctgctgcc 721 aacccttctc cgggaacaag gaacagggtt acatgagcct caaggagaac cagatctgcg 781 tgggcgtggg cagaagcaag gatgctgacg gcttcatccg cgtcagcagt ggcaagaagc 841 ggggcctggt gccagtcgac gccctgactg agatcttgcc aactttcttg tacaaagtgg 901 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 961 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta