Construct: ORF ccsbBroad304_13661
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004923.1_s304c1, BRDN0000460196
- Derived from:
- ccsbBroadEn_13661
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C9orf153 (389766)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13661
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 389766 | C9orf153 | chromosome 9 open reading f... | NM_001276366.2 | 100% | 100% | |
| 2 | human | 389766 | C9orf153 | chromosome 9 open reading f... | NM_001276367.2 | 100% | 100% | |
| 3 | human | 389766 | C9orf153 | chromosome 9 open reading f... | NM_001276368.1 | 100% | 100% |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 354
- ORF length:
- 285
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gttcctcact ggagacacca gtccagctga ggacaataga gaagccaccc 121 ttcctcaatg ttcacttcca gaattatatg catgtattga gaattttaat aaggagagca 181 agaaatcaaa tcttctaaaa atgcatggta tttcacttaa cgaagcacag gaagtacttg 241 ctagaaacct gaatgtcatg tcattcacca ggggcgctga tgtgagagga gatctccaac 301 ctgttatcag gaaaacaatt catgaatcta agggatctgg aatggagata tttttgctca 361 actttcttgt acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct 421 acgtagtaat gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt 481 a