Construct: ORF ccsbBroad304_13679
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF005198.1_s304c1, BRDN0000398671
- Derived from:
- ccsbBroadEn_13679
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- C16orf74 (404550)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13679
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 404550 | C16orf74 | chromosome 16 open reading ... | NM_206967.3 | 84.2% | 84.2% | 1_36del |
| 2 | human | 404550 | C16orf74 | chromosome 16 open reading ... | NR_161454.1 | 21.3% | (many diffs) | |
| 3 | human | 404550 | C16orf74 | chromosome 16 open reading ... | NR_161452.1 | 20% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 258
- ORF length:
- 192
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgtg tgtcagcagc agcagcagca gccacgacga ggcccccgtc ctgaacgaca 121 agcacctgga cgtgcccgac atcatcatca cgccccccac ccccacgggc atgatgctgc 181 cgagggactt ggggagcaca gtctggctgg atgagacagg gtcgtgccca gatgatggag 241 aaatcgaccc agaagcctgc ccaactttct tgtacaaagt ggttggtaag cctatcccta 301 accctctcct cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta 361 agtcgacaat caacctctgg atta