Transcript: Human NM_206967.3

Homo sapiens chromosome 16 open reading frame 74 (C16orf74), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-05-02
Taxon:
Homo sapiens (human)
Gene:
C16orf74 (404550)
Length:
911
CDS:
190..420

Additional Resources:

NCBI RefSeq record:
NM_206967.3
NBCI Gene record:
C16orf74 (404550)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_206967.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000268855 TCGTGCCCAGATGATGGAGAA pLKO_005 382 CDS 100% 4.050 5.670 N C16orf74 n/a
2 TRCN0000283806 CTGGAGCAGGTGTGCTGAATA pLKO_005 494 3UTR 100% 13.200 9.240 N C16orf74 n/a
3 TRCN0000268812 GCTTTCAAATGTGTGTCAGCA pLKO_005 218 CDS 100% 2.640 1.848 N C16orf74 n/a
4 TRCN0000283808 CATGATGCTGCCGAGGGACTT pLKO_005 330 CDS 100% 1.350 0.945 N C16orf74 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_206967.3, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13679 pDONR223 100% 84.2% 84.2% None 1_36del n/a
2 ccsbBroad304_13679 pLX_304 0% 84.2% 84.2% V5 1_36del n/a
3 TRCN0000472199 CCATTTAGCGATTAAATAACAGAA pLX_317 100% 84.2% 84.2% V5 1_36del n/a
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