Construct: ORF ccsbBroad304_13711
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007564.1_s304c1, BRDN0000411589
- Derived from:
- ccsbBroadEn_13711
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- BOLA2 (552900)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13711
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 552900 | BOLA2 | bolA family member 2 | NM_001320579.1 | 81.3% | 70.6% | (many diffs) |
| 2 | human | 552900 | BOLA2 | bolA family member 2 | NM_001031827.2 | 80.9% | 78.9% | (many diffs) |
| 3 | human | 107282092 | BOLA2-SMG1P6 | BOLA2-SMG1P6 readthrough | NM_001320623.1 | 43.3% | 42% | (many diffs) |
| 4 | human | 654483 | BOLA2B | bolA family member 2B | NM_001039182.4 | 37.5% | 35.5% | (many diffs) |
| 5 | human | 107282092 | BOLA2-SMG1P6 | BOLA2-SMG1P6 readthrough | NM_001320622.1 | 35% | 31.4% | 260_261ins38;335_915del |
| 6 | human | 107282092 | BOLA2-SMG1P6 | BOLA2-SMG1P6 readthrough | NM_001320624.1 | 34.5% | 31.8% | (many diffs) |
| 7 | human | 654483 | BOLA2B | bolA family member 2B | NM_001320607.2 | 32.6% | 21% | (many diffs) |
| 8 | human | 107282092 | BOLA2-SMG1P6 | BOLA2-SMG1P6 readthrough | NR_135316.1 | 20.9% | (many diffs) | |
| 9 | mouse | 66162 | Bola2 | bolA-like 2 (E. coli) | NM_175103.3 | 33.4% | 32.8% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 441
- ORF length:
- 372
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggcaagcgcg aaaagcctgg accgctggaa agcccggctg cttgagggcg 121 gaagtactgc gttgacgtac gccttagtaa gggcggaagt gagttttcca gcggaagtgg 181 ctcctgtaag gcagcaaggt agcgtggccg gcgcccgagc tggggttgtg tccctgctgg 241 gctgccgttc cagctggact gccgccatgg aactcagcgc cgaatacctc cgcgagaagc 301 tgcagcggga cctggaggcg gagcatgtgg aggtggagga cacgaccctc aaccgttgct 361 cctgtagctt ccgagtcctg gtggtgtcgg ccaagttcga ggggaaaccg ctgcttcaga 421 gacacaggtt ctgtacagag ttgccaactt tcttgtacaa agtggttggt aagcctatcc 481 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 541 ttaagtcgac aatcaacctc tggatta