Transcript: Human NM_001320624.1

Homo sapiens BOLA2-SMG1P6 readthrough (BOLA2-SMG1P6), transcript variant 3, mRNA.

Source:
NCBI, updated 2019-06-08
Taxon:
Homo sapiens (human)
Gene:
BOLA2-SMG1P6 (107282092)
Length:
1885
CDS:
514..1371

Additional Resources:

NCBI RefSeq record:
NM_001320624.1
NBCI Gene record:
BOLA2-SMG1P6 (107282092)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001320624.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000262898 ATGGAACTCAGCGCCGAATAC pLKO_005 712 CDS 100% 10.800 5.400 Y BOLA2B n/a
2 TRCN0000255857 CATGGAACTCAGCGCCGAATA pLKO_005 711 CDS 100% 10.800 5.400 Y BOLA2 n/a
3 TRCN0000255855 GTTGACGTACGCCTTAGTAAG pLKO_005 576 CDS 100% 10.800 5.400 Y BOLA2 n/a
4 TRCN0000180088 CGTTGACGTACGCCTTAGTAA pLKO.1 575 CDS 100% 5.625 2.813 Y BOLA2 n/a
5 TRCN0000282459 GCGTTGACGTACGCCTTAGTA pLKO_005 574 CDS 100% 5.625 2.813 Y BOLA2B n/a
6 TRCN0000262899 AGTAAGGGCGGAAGTGAGTTT pLKO_005 591 CDS 100% 4.950 2.475 Y BOLA2B n/a
7 TRCN0000183297 GCTTGAAGAATATTCCTGTTT pLKO.1 1142 CDS 100% 4.950 2.475 Y BOLA2 n/a
8 TRCN0000255856 GGAAGTACTGCGTTGACGTAC pLKO_005 565 CDS 100% 4.050 2.025 Y BOLA2 n/a
9 TRCN0000262897 TCCTGTAAGGCAGCAAGGTAG pLKO_005 627 CDS 100% 4.050 2.025 Y BOLA2B n/a
10 TRCN0000255854 AGCGGAAGTGGCTCCTGTAAG pLKO_005 615 CDS 100% 3.600 1.800 Y BOLA2 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001320624.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_13711 pDONR223 100% 34.5% 31.8% None (many diffs) n/a
2 ccsbBroad304_13711 pLX_304 0% 34.5% 31.8% V5 (many diffs) n/a
3 TRCN0000475372 GATGCAATGCTAAGATCTATCTCA pLX_317 100% 34.5% 31.8% V5 (many diffs) n/a
4 TRCN0000488255 ACGTTGGAAAGGGAAAGCTGGCTT pLX_317 1.7% 7% 5.6% V5 (not translated due to prior stop codon) (many diffs) n/a
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