Construct: ORF ccsbBroad304_13747
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF004271.1_s304c1, BRDN0000403339
- Derived from:
- ccsbBroadEn_13747
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- MOSMO (730094)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13747
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 730094 | MOSMO | modulator of smoothened | NM_001164579.2 | 50.7% | 43.7% | (many diffs) |
| 2 | mouse | 233812 | BC030336 | cDNA sequence BC030336 | XM_006507689.2 | 56.7% | 51% | (many diffs) |
| 3 | mouse | 233812 | BC030336 | cDNA sequence BC030336 | NM_001164580.1 | 49% | 43.7% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 339
- ORF length:
- 273
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggg ttacttctgc cagattaatc tgggaagaaa cagagcactc actgtgggcc 121 tcgtgcgaca gtgtcaaaca atccatggac gagaccggac gtgcatccct ccccggcttc 181 ccccggagtg ggtcaccaca ctgtttttta tcatcatggg aatcatttca ttgactgtca 241 catgtggttt gctggtggct tcccactggc gaagagaagc tacaaaatat gctcgatgga 301 tagcattcac tggaaagtgt gtgcattctt ctcataccta cccaactttc ttgtacaaag 361 tggttggtaa gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta 421 gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg gatta