Construct: ORF ccsbBroad304_13814

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF014312.1_s304c1, BRDN0000401929
Derived from:
ccsbBroadEn_13814
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
ARHGAP5 (394)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13814
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 394 ARHGAP5 Rho GTPase activating prote... NM_001030055.2 99.9% 12.7% (many diffs)
2 human 394 ARHGAP5 Rho GTPase activating prote... XM_005267635.4 99.9% 12.7% (many diffs)
3 human 394 ARHGAP5 Rho GTPase activating prote... XM_005267636.4 99.9% 12.7% (many diffs)
4 human 394 ARHGAP5 Rho GTPase activating prote... NM_001173.3 99.8% 12.7% (many diffs)
5 human 394 ARHGAP5 Rho GTPase activating prote... XM_017021288.1 99.8% 12.7% (many diffs)
6 mouse 11855 Arhgap5 Rho GTPase activating prote... NM_009706.2 93.4% 12.4% (many diffs)
7 mouse 11855 Arhgap5 Rho GTPase activating prote... XM_006515438.3 93.4% 12.4% (many diffs)
8 mouse 11855 Arhgap5 Rho GTPase activating prote... XM_011243989.1 93.4% 12.4% (many diffs)
9 mouse 11855 Arhgap5 Rho GTPase activating prote... XM_011243990.1 93.4% 12.4% (many diffs)
10 mouse 11855 Arhgap5 Rho GTPase activating prote... XM_006515439.2 93.3% 12.4% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
648
ORF length:
579
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat gatggcaaaa aacaaagagc ctcgtccccc atcctatacc atcagtatag
121ttggactctc tgggactgaa aaagacaaag gtaactgtgg agttggaaag tcttgtttgt
181gcaatagatt tgtacgctca aaagcagatg aatattatcc agagcatact tctgtgctta
241gcaccattga ctttggagga cgagtagtaa acaatgatca ctttttgtac tggggtgaca
301taatacaaaa tagtgaagat ggagtagaat gcaaaattca tgtcattgaa caaacagagt
361tcattgatga ccagactttc ttgcctcatc ggagtacgaa tttgcaacca tatataaaac
421gtgcagctgc atctaaattg cagtcagcag aaaaactaat gtacatttgc actgatcagc
481taggcttaga acaagacttt gaacagaagc aaatgcctga agggaagctc aacgtagatg
541gatttttatt atgcattgat gtaagtcaag gatgcaatag gaagtttgat gatcaactta
601aatttgtgaa taaccttttt gtccagttat caaaatcaaa aaacctgtaa taatagcagc
661aactaaatgt gatgaatgcg tggatcatta tcttagagaa gttcaggcat ttgcttcaaa
721taaaaagaac cttcttgtag tggaaacatc agcacgattt aatgtcaaca ttgaaacatg
781ttttactgca ctggtacaaa tgttggataa aactcgtagc aagcctaaaa ttattcccta
841tttggatgct tataaaacac agagacaact tgttgtcaca gcaacagata agtttgaaaa
901acttgtgcag actgtgagag attatcatgc aacttggaaa actgttagta ataaattaaa
961aaatcatcct gattatgaag aatacatcaa cttagaggga acaagaaagg ccagaaatac
1021attctcaaaa catatagaac aacttaaaca ggaacatata agaaaaagga gagaagagta
1081tataaatact ttaccaagag cttttaacac tcttttgcca aatctagaag agattgaaca
1141tttgaattgg tcagaagctt tgaagttaat ggaaaagaga gcagatttcc agttatgttt
1201tgtggtgcta gaaaaaactc cttgggatga aactgaccat atagacaaaa ttaatgatag
1261gcggattcca tttgacctcc tgagcacttt agaagctgaa aaagtctatc agaaccatgt
1321acagcatctg atatccgaga agaggagggt ggaaatgaag gaaaaattca aaaagacttt
1381ggaaaaaatt caattcattt caccagggca gccatgggag gaagttatgt gctttgttat
1441ggaggatgaa gcctacaaat atatcactga ggctgatagc aaagaggtat atggtaggca
1501tcagcgagaa atagttgaaa aagccaaaga agagtttcaa gaaatgcttt ttgagcattc
1561tgaacttttt tatgatttag atcttaatgc aacacctagt tcagataaaa tgagtgaaat
1621tcatacagtt ctgagtgaag aacctagata taaagcttta cagaaacttg cacctgatag
1681ggaatccctt ctacttaagc atataggatt tgtttatcat cccactaaag aaacatgtct
1741tagtggccaa aattgtacag acattaaagt ggagcagtta cttgctagta gtcttttaca
1801gttggatcat ggccgcttaa gattatatca cgatagtacc aatatagata aagttaacct
1861ttttatttta gggaaggatg gccttgccca agaactagca aatgagataa ggacacaatc
1921cactgatgat gagtatgcct tagatggaaa aatttatgaa cttgatcttc ggccggttga
1981tgccaaatcg ccttactttt tgagtcagtt atggactgcc gcctttaaac cacatgggtg
2041cttctgtgta tttaattcca ttgagtcatt gagttttatt ggggaattta ttgggaaaat
2101aagaactgaa gcttctcaga tcagaaaaga taaatacatg gctaatcttc catttacatt
2161aattctggct aatcagagag attccattag taagaatcta ccaattctca ggcaccaagg
2221gcagcagttg gcaaacaagt tgcaatgtcc ttttgtagat gtacctgctg gtacatatcc
2281tcgtaaattt aatgaaaccc agataaagca agctctcaga ggagtattgg aatcagttaa
2341acacaatttg gatgtggtga gcccaattcc tgccaataag gacttatcag aagctgactt
2401gagaattgtc atgtgcgcca tgtgtggaga tccatttagt gtggatctta ttctttcacc
2461cttccttgat tctcattctt gcagtgctgc tcaagctgga cagaataatt ccctaatgct
2521tgataaaatc attggtgaaa aaaggaggcg aatacagatc acaatattat cgtaccactc
2581ttcaattgga gtaagaaaag atgaactagt tcatgggtat atattagttt actctgcaaa
2641acggaaagct tcgatgggaa tgcttcgagc atttctatca gaagttcaag acaccattcc
2701tgtacagctg gtggcagtta ctgacagcca agcagatttt tttgaaaatg aggctatcaa
2761agagttaatg actgaaggag aacacattgc aactgagatc actgctaaat ttacagcact
2821gtattcttta tctcagtatc atcggcaaac tgaggtcttt actctgtttt ttagtgatgt
2881tctagagaaa aaaaatatga tagaaaattc ttatttgtct gataatacaa gggaatcaac
2941ccatcaaagt gaagatgttt ttctaccatc tcccagagac tgttttccct ataataacta
3001ccctgattca gatgatgaca cagaagcacc acctccttat agtccaattg gggatgatgt
3061acagttgctc ccaacaccta gtgaccgttc cagatataga ttagatttgg aaggaaatga
3121gtatcctatt catagtaccc caaactgtca tgaccatgaa cgcaaccata aagtgcctcc
3181acctattaaa cctaaaccag ttgtacctaa gacaaatgtg aaaaaactcg atccaaacct
3241tttaaaaaca attgaagctg gtattggtaa aaatccaaga aagcagactt cccgggtgcc
3301tttggcacat cctgaagata tggatccttc agataactat gcggaaccca ttgatacaat
3361tttcaaacag aagggctatt ctgatgagat ttatgttgtc ccagatgata gtcaaaatcg
3421tattaaaatt cgaaactcat ttgtaaataa cacccaagga gatgaagaaa atgggttttc
3481tgatagaacc tcaaaaagtc atggggaacg gaggccttca aaatacaaat ataaatctaa
3541aaccttgttt agtaaagcca agtcatacta tagaagaaca cattcagatg ccagtgatga
3601tgaggctttc accacttcta aaacaaaaag aaaaggaaga catcgtggaa gtgaagaaga
3661tccacttctt tctcctgttg aaacttggaa aggtggtatt gataatcctg caatcacttc
3721tgaccaggag ttagatgata agaagatgaa gaagaaaacc cacaaagtga aagaagataa
3781aaagcagaaa aagaaaacta agaacttcaa tccaccaaca cgtagaaatt gggaaagtaa
3841ttactttggg atgcccctcc aggatctggt tacagctgag aagcccatac cactatttgt
3901tgagaaatgt gtggaattta ttgaagatac agggttatgt accgaaggac tctaccgtgt
3961cagcgggaat aaaactgacc aagacaatat tcaaaagcag tttgatcaag atcataatat
4021caatctagtg tcaatggaag taacagtaaa tgctgtagct ggagccctta aagctttctt
4081tgcagatctg ccagatcctt taattccata ttctcttcat ccagaactat tggaagcagc
4141aaaaatcccg gataaaacag aacgtcttca tgccttgaaa gaaattgtta agaaatttca
4201tcctgtaaac tatgatgtat tcagatacgt gataacacat ctaaacaggg ttagtcagca
4261acataaaatc aacctaatga cagcagacaa cttatccatc tgtttttggc caaccttgat
4321gagacctgat tttgaaaatc gagagtttct gtctactact aagattcatc aatctgttgt
4381tgaaacattc attcagcagt gtcagttttt cttttacaat ggagaaattg tagaaacgac
4441aaacattgtg gctcctccac caccttcaaa cccaggacag ttggtggaac caatggtgcc
4501acttcagttg ccgccaccat tgcaacctca gctgatacaa ccacaattac aaacggatcc
4561tcttggtatt atattgccaa ctttcttgta caaagtggtt ggtaagccta tccctaaccc
4621tctcctcggt ctcgattcta cgtagtaatg agctagcgct aaccggtggc gcgttaagtc
4681gacaatcaac ctctggatta

Download FASTA (ORF) (Full)

GPP Web Portal Terms of Service

Effective Date: December 8, 2025
By using this site, you agree to our terms and conditions below.

Overview of Terms

The data made available on this website were generated for research purposes and are not intended for clinical or commercial uses. Commercial use (or other use for profit-making purposes) of the GPP Web Portal and its tools, is not permitted under these terms and may require a separate license agreement from Broad or its contributors. For more information, please contact partnering@broadinstitute.org.

The original data may be subject to rights claimed by third parties, including but not limited to, patent, copyright, other intellectual property rights, biodiversity-related access and benefit-sharing rights. It is the responsibility of users of Broad Institute services to ensure that their use of the data does not infringe any of the rights of such third parties.

Any questions or comments concerning these Terms of Use can be addressed to: legal@broadinstitute.org.

By accessing and viewing this GPP Web Portal, you agree to the following terms and conditions:

Attribution

You agree to acknowledge the Broad Institute (e.g., in publications, services or products) for any of your use of its online services, databases or software in accordance with good scientific practice. You agree to use the acknowledgment wording provided for the relevant tools as indicated on the FAQ for each tool.

Updating the Terms of Use

We reserve the right to update these Terms of Use at any time. When alterations are inevitable, we will attempt to give reasonable notice of any changes by placing a notice on our website, but you may wish to check each time you use the website. The date of the most recent revision will appear on this, the "GPP Web Portal Terms of Use" page. If you do not agree to these changes, please do not continue to use our online services. We will also make available an archived copy of the previous Terms of Use for comparison.

Indemnification and Disclaimer of Warranties

You are using this GPP Web Portal at your own risk, and you hereby agree to hold Broad and its contributors and their trustees, directors, officers, employees, and affiliated investigators harmless for any third party claims which may arise from your use of the GPP Web Portal, the tools available therein, or any portion thereof. Further, you agree to indemnify Broad, its contributors, and its and their trustees, directors, officers, employees, affiliated investigators, students, and affiliates for any loss, costs, claims, damages, or other liabilities arising from any unpermitted commercial or profit-making use you make of the GPP Web Portal. The GPP Web Portal is a research tool and is provided "as is". Broad does not represent that the GPP Web Portal is free of errors or bugs or suitable for any particular tasks.

ANY EXPRESS OR IMPLIED WARRANTIES, INCLUDING, BUT NOT LIMITED TO, THE IMPLIED WARRANTIES OF MERCHANTABILITY, FITNESS FOR A PARTICULAR PURPOSE, NONINFRINGEMENT, OR THE ABSENCE OF LATENT OR OTHER DEFECTS ARE DISCLAIMED. IN NO EVENT SHALL BROAD OR ITS CONTRIBUTORS BE LIABLE FOR ANY DIRECT, INDIRECT, INCIDENTAL, SPECIAL, EXEMPLARY, OR CONSEQUENTIAL DAMAGES HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY, WHETHER IN CONTRACT, STRICT LIABILITY, OR TORT (INCLUDING NEGLIGENCE OR OTHERWISE) ARISING IN ANY WAY OUT OF THE USE OF THE GPP WEB PORTAL, EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE.

Governing Law

The terms and conditions herein shall be construed, governed, interpreted, and applied in accordance with the internal laws of the Commonwealth of Massachusetts, U.S.A. Furthermore, by accessing, downloading, or using the Database, You consent to the personal jurisdiction of, and venue in, the state and federal courts within Massachusetts with respect to Your download or use of the Database.