Construct: ORF ccsbBroad304_13818
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF001939.1_s304c1, BRDN0000460162
- Derived from:
- ccsbBroadEn_13818
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- ATP5F1E (514)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13818
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 514 | ATP5F1E | ATP synthase F1 subunit eps... | NM_006886.4 | 99.3% | 66.6% | 103_104insA |
| 2 | human | 100533975 | SLMO2-ATP5E | SLMO2-ATP5E readthrough | NR_037930.1 | 18.7% | (many diffs) | |
| 3 | human | 100533975 | SLMO2-ATP5E | SLMO2-ATP5E readthrough | NR_037929.1 | 14% | (many diffs) | |
| 4 | mouse | 67126 | Atp5e | ATP synthase, H+ transporti... | NM_025983.3 | 87.2% | 63.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 171
- ORF length:
- 105
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggt ggcctactgg agacaggctg gactcagcta catccgatac tcccagatct 121 gtgcaaaagc agtgagagat gcactgaaga cagaattcaa agcaaatgac tgagaagact 181 tctggcagca acgtaaaaat tgtgaaagta aagaaggaat acccaacttt cttgtacaaa 241 gtggttggta agcctatccc taaccctctc ctcggtctcg attctacgta gtaatgagct 301 agcgctaacc ggtggcgcgt taagtcgaca atcaacctct ggatta