Construct: ORF ccsbBroad304_13865
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF003340.1_s304c1, BRDN0000461669
- Derived from:
- ccsbBroadEn_13865
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Has stop codon in insert
Originally Annotated References:
- Gene:
- GSPT1 (2935)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13865
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 2935 | GSPT1 | G1 to S phase transition 1 | NM_001130006.2 | 99.4% | 99.2% | 16_24delGGCGGCGGC;274G>T;299T>C |
| 2 | human | 2935 | GSPT1 | G1 to S phase transition 1 | NM_002094.4 | 99.2% | 99% | (many diffs) |
| 3 | human | 23708 | GSPT2 | G1 to S phase transition 2 | NM_018094.5 | 85.3% | 86.2% | (many diffs) |
| 4 | human | 2935 | GSPT1 | G1 to S phase transition 1 | NM_001130007.2 | 78.5% | 78.5% | 0_1ins405;22_24delGTA |
| 5 | mouse | 14852 | Gspt1 | G1 to S phase transition 1 | NM_001130008.1 | 90.8% | 94.5% | (many diffs) |
| 6 | mouse | 14852 | Gspt1 | G1 to S phase transition 1 | NM_146066.2 | 90.7% | 94.3% | (many diffs) |
| 7 | mouse | 14853 | Gspt2 | G1 to S phase transition 2 | NM_008179.2 | 80.8% | 81.9% | (many diffs) |
| 8 | mouse | 77633 | 4930594C11Rik | RIKEN cDNA 4930594C11 gene;... | NR_024017.1 | 38.3% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1965
- ORF length:
- 1899
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga tccgggcagt ggcggcggcg gcggcggcgg cgggagcagc agcggcagca 121 gcagcagcga ctcggcgcct gactgctggg accaggcgga catggaagcc cccgggccgg 181 gcccttgcgg cggcggcggc tccctggcgg cggcggccga ggcccagcgg gagaacctca 241 gcgcggcctt cagccggcaa ctcaacgtca acgccaagcc cttcgtgccc aacgtccacg 301 ccgccgagtt cgtgccgtcc ttcctgcggt gcccggcagc gccgccaccc ccagctggcg 361 gcgccgccaa taaccacgga gccggcagcg gcgcgggagg ccgtgcggca cctgtggaat 421 cctctcaaga ggaacagtca ttgtgtgaag gttcaaattc agctgttagc atggaacttt 481 cagaacctat tgaaaatgga gagacagaaa tgtctccaga agaatcatgg gagcacaaag 541 aagaaataag tgaagcagag ccagggggtg gttccttggg agatggaagg ccgccagagg 601 aaagtgccca tgaaatgatg gaggaggaag aggaaatccc aaaacctaag tctgtggttg 661 caccgccagg tgctcctaag aaagagcatg taaatgtagt attcattggg cacgtagatg 721 ctggcaagtc aaccattgga ggacaaataa tgtatttgac tggaatggtt gacaaaagga 781 cgcttgaaaa gtatgaaaga gaagctaaag agaaaaacag agaaacttgg tacttgtctt 841 gggccttaga cacaaatcag gaagaacgag acaagggtaa aacagtagaa gtgggtcgtg 901 cctattttga aaccgaaaag aagcatttca caattctaga tgcccctggc cacaagagtt 961 ttgtcccaaa tatgattggt ggtgcctctc aagctgattt ggctgtgctg gtaatctcag 1021 ccaggaaagg agagtttgaa actggatttg aaaaaggagg acagacaaga gaacatgcaa 1081 tgttggcaaa gacagcaggt gtaaaacacc taattgtgct aattaataag atggatgatc 1141 caacagtaaa ttggagcaat gagagatatg aagaatgtaa ggagaaacta gtgccatttt 1201 tgaaaaaagt tggcttcaat cccaaaaagg acattcactt tatgccctgc tcaggactta 1261 ctggagcaaa tctcaaagag cagtcggatt tctgtccttg gtacattgga ttaccgttta 1321 ttccatatct ggataatttg ccgaacttca atagatcagt tgatggacca atcaggctgc 1381 caattgtgga taagtacaag gatatgggca ctgtggtcct gggaaagctg gaatcaggat 1441 ctatttgtaa aggccagcag cttgtgatga tgccaaacaa gcacaacgtg gaagttcttg 1501 gaatactttc cgatgatgta gagactgata ccgtagcccc aggtgaaaac ctcaaaatca 1561 gactgaaagg aattgaagaa gaggagattc ttccagggtt tatactttgt gatcctaata 1621 atctttgtca ttctggacgc acatttgatg cccagatagt gattatagag cacaaatcca 1681 tcatctgccc aggctataat gcggtgctgc atattcatac ctgtattgag gaggtggaaa 1741 taacagcctt aatctgcttg gtagacaaaa aatcaggaga aaaaagtaag acccgacccc 1801 gttttgtcaa acaagatcaa gtatgcattg ctcgcttaag gacagcagga accatctgcc 1861 ttgagacctt taaagacttc cctcagatgg gtcgtttcac cttaagagat gagggtaaga 1921 ccattgcaat tggaaaagtt ctgaaactgg ttccagagaa agactaagct attttcttgt 1981 acaaagtggt tggtaagcct atccctaacc ctctcctcgg tctcgattct acgtagtaat 2041 gagctagcgc taaccggtgg cgcgttaagt cgacaatcaa cctctggatt a