Construct: ORF ccsbBroad304_13908

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF015685.1_s304c1, BRDN0000460243
Derived from:
ccsbBroadEn_13908
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
MYL5 (4636)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_13908
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
n/a

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 4636 MYL5 myosin light chain 5 NM_001363650.2 99.4% 91% 243G>A;361_362insG
2 human 4636 MYL5 myosin light chain 5 NM_002477.1 75.9% 69.7% 1_123del;366G>A;484_485insG
3 human 4636 MYL5 myosin light chain 5 XM_017008245.1 75.9% 69.7% 1_123del;366G>A;484_485insG
4 human 4636 MYL5 myosin light chain 5 XM_017008246.1 75.9% 69.7% 1_123del;366G>A;484_485insG
5 human 4636 MYL5 myosin light chain 5 XM_006713886.2 40.1% 36.9% 1_588del;831G>A;949_950insG
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
465
ORF length:
399
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgga tcagaaccga gatggcttca ttgacaagga ggacctgaag gacacctatg
121cctccctggg caagaccaac gtcaaggacg acgagctgga cgccatgctc aaagaggcct
181cggggcccat caacttcacc atgtttctga acctgtttgg ggagaagctg agcggtaccg
241acgccgagga gaccattctt aacgccttca agatgctgga cccggacggg aaagggaaaa
301tcaacaaaga gtacatcaag cgtctgctga tgtcccaggc tgacaagatg acggcggaag
361aggtggacca gatgttccag ttcgcctcca tcgatgtggc gggcaacctg gactacaagg
421cgctcaggct acgtgatcac ccacggggag gagaaggagg agtgcccaac tttcttgtac
481aaagtggttg gtaagcctat ccctaaccct ctcctcggtc tcgattctac gtagtaatga
541gctagcgcta accggtggcg cgttaagtcg acaatcaacc tctggatta

Download FASTA (ORF) (Full)

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