Construct: ORF ccsbBroad304_13959
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008568.1_s304c1, BRDN0000401513
- Derived from:
- ccsbBroadEn_13959
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- SP3 (6670)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13959
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 6670 | SP3 | Sp3 transcription factor | NM_003111.4 | 99.9% | 99.7% | 2337delA |
| 2 | human | 6670 | SP3 | Sp3 transcription factor | NM_001172712.1 | 99.5% | 99.3% | 153_154insCAGGACACT;2328delA |
| 3 | human | 6670 | SP3 | Sp3 transcription factor | NM_001017371.5 | 91.2% | 91% | 0_1ins204;2133delA |
| 4 | mouse | 20687 | Sp3 | trans-acting transcription ... | NM_001018042.3 | 93.9% | 95.6% | (many diffs) |
| 5 | mouse | 20687 | Sp3 | trans-acting transcription ... | NM_001098425.1 | 86.5% | 88.1% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2412
- ORF length:
- 2343
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gaccgctccc gaaaagcccg tgaaacaaga ggaaatggct gccttggacg 121 tggatagcgg cggcggcggt ggcggcggcg gcggccacgg cgagtatctg cagcagcagc 181 aacagcacgg aaacggtgcg gtggcggcgg cagcggcggc ccaggacact cagccgtcac 241 cgctcgctct gctggccgct acctgcagca agatagggcc gccatcgccg ggcgacgacg 301 aggaggaggc ggccgccgca gccggggccc ccgccgccgc cggagcgaca ggtgatttgg 361 cttctgcaca gttaggagga gcaccaaacc gatgggaggt tttgtcagcc acacctacaa 421 ctataaaaga tgaagctggt aatctagtcc agattccaag tgctgctact tcaagtgggc 481 agtatgttct tccccttcag aatttgcaga atcaacaaat attttccgtt gcaccaggat 541 cagattcatc aaatggtaca gtgtccagtg ttcaatatca agtgatacca cagatccagt 601 cagcagatgg tcagcaggtt caaattggtt tcacaggctc ttcagataat gggggtataa 661 atcaagaaag cagtcaaatt cagatcattc ctggctctaa tcaaacctta cttgcctctg 721 gaacaccttc tgctaacatc cagaatctca taccacagac tggtcaagtc caggttcagg 781 gagttgcaat tggtggttca tcttttcctg gtcaaaccca agtagttgct aatgtgcctc 841 ttggtctgcc aggaaatatt acgtttgtac caatcaatag tgtcgatcta gattctttgg 901 gactctcggg cagttctcag acaatgactg caggcattaa tgccgacgga catttgataa 961 acacaggaca agctatggat agttcagaca attcagaaag gactggtgag cgggtttctc 1021 ctgatattaa tgaaactaat actgatacag atttatttgt gccaacatcc tcttcatcac 1081 agttgcctgt tacgatagat agtacaggta tattacaaca aaacacaaat agcttgacta 1141 catctagtgg gcaggttcat tcttcagatc ttcagggaaa ttatatccag tcgcctgttt 1201 ctgaagagac acaggcacag aatattcagg tttctacagc acagcctgtt gtacagcatc 1261 tacaacttca agagtctcag cagccaacca gtcaagccca aattgtgcaa ggtattacac 1321 cacagacaat ccatggtgtg caagccagtg gtcaaaatat atcacaacag gctttgcaaa 1381 atcttcagtt gcagctgaat cctggaacct ttttaattca ggcacagaca gtgacccctt 1441 ctggacaggt aacttggcaa acgtttcaag tacaaggggt ccagaacttg cagaatttgc 1501 aaatacagaa tactgctgcc caacaaataa ctttgacgcc tgttcaaacc ctcacacttg 1561 gtcaagttgc ggcaggtgga gccttcactt caactccagt tagtctaagc actggtcagt 1621 tgccaaatct acaaacagtt acagtgaact ctatagattc tgctggtata cagctacatc 1681 caggagagaa tgctgacagt cctgcagata ttaggatcaa ggaagaagaa cctgatcctg 1741 aagagtggca gctcagtggt gattctacct tgaataccaa tgacctaaca cacttaagag 1801 tacaggtggt agatgaagaa ggggaccaac aacatcaaga aggaaaaaga cttcggaggg 1861 tagcttgcac ctgtcccaac tgtaaagaag gtggtggaag aggtaccaat cttgggaaaa 1921 agaagcaaca catttgtcat ataccaggat gtggtaaagt ctatgggaag acctcacatc 1981 tgagagctca tctgcgttgg cattctggag aacgcccttt tgtttgtaac tggatgtact 2041 gtggtaaaag atttactcga agtgatgaat tacagaggca cagaagaaca catacaggtg 2101 agaagaaatt tgtttgtcca gaatgttcaa aacgctttat gagaagtgac caccttgcca 2161 aacatattaa aacacaccag aataaaaaag gtattcactc tagcagtaca gtgctggcat 2221 ctgtggaagc tgcgcgagat gatactttga ttactgcagg aggaacaacg cttatccttg 2281 caaatattca acaaggttct gtttcaggga taggaactgt taatacttcc gccaccagca 2341 atcaagatat ccttaccaac actgaaatac ctttacagct tgtcacagtt tctggaaatg 2401 agacatggag ttgccaactt tcttgtacaa agtggttggt aagcctatcc ctaaccctct 2461 cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac 2521 aatcaacctc tggatta