Construct: ORF ccsbBroad304_13997
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF014528.1_s304c1, BRDN0000399471
- Derived from:
- ccsbBroadEn_13997
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- CBX4 (8535)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_13997
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 8535 | CBX4 | chromobox 4 | NM_003655.3 | 51.7% | 51.6% | 379_1188del;1671C>N |
| 2 | human | 8535 | CBX4 | chromobox 4 | XM_011525399.2 | 39.9% | 39.8% | 0_1ins198;181_990del;1473C>N |
| 3 | mouse | 12418 | Cbx4 | chromobox 4 | NM_007625.2 | 45.9% | 46.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 936
- ORF length:
- 870
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgga gctgccagct gttggcgagc acgtcttcgc ggtggagagc atcgagaaga 121 agcggatccg caagggcaga gtggagtatc tggtgaaatg gagaggctgg tcgcccaaat 181 ataacacgtg ggaaccggag gagaacatcc tggaccccag gctgctgatc gccttccaga 241 acagggaacg gcaggagcag ctgatgggat atcggaagag agggccgaag cccaaaccgc 301 tagtggtgca ggtgcctacc tttgcccgtc gttccaatgt cctgaccggc ctccaggact 361 cctccactga caaccgtgcc aagctggatt tgggcgcgca ggggaagggc caggggcatc 421 agtacgagct caacagcaag aagcaccacc accacgccgt cggcctgaat ctctcccacg 481 tgcgcaagcg ctgcctctcc gagacccacg gcgagcgcga gccctgcaag aagcggctga 541 ctgcgcgcag catcagcacc cccacctgcc tggggggcag cccagccgct gagcgcccgg 601 ccgacctgcc accagccgcc gccctcccgc agcccgaggt catcctgcta gactcagacc 661 tggatgaacc catagacttg cgctgcgtca agacgcgcag cgaggccggg gagccgccca 721 gctccctcca ggtgaagccc gagacaccgg cgtcggcggc ggtggcggtg gcggcggcag 781 cggcacccac cacgacggcg gagaagcctc cagccgaggc ccaggacgaa cctgcagagt 841 cgctgagcga gttcaagccc ttctttggga atataattat caccgacgtc accgcgaact 901 gcctcaccgt tactttcaag gagtangtga cggtgtaccc aactttcttg tacaaagtgg 961 ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa tgagctagcg 1021 ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta