Construct: ORF ccsbBroad304_14058
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF000995.1_s304c1, BRDN0000460659
- Derived from:
- ccsbBroadEn_14058
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- DHRS4 (10901)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14058
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- n/a
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | NM_021004.4 | 99.8% | 9delG | |
| 2 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | NM_001282988.2 | 87.6% | 9delG;305_306ins102 | |
| 3 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | XM_006720005.3 | 83.6% | 9delG;531_532ins135 | |
| 4 | human | 728635 | DHRS4L1 | dehydrogenase/reductase 4 l... | XM_006720246.3 | 81.5% | (many diffs) | |
| 5 | human | 317749 | DHRS4L2 | dehydrogenase/reductase 4 l... | NM_198083.4 | 81% | (many diffs) | |
| 6 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | NM_001282990.2 | 71.4% | 9delG;305_306ins102;429_430ins135 | |
| 7 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | NM_001282989.2 | 68.9% | 9delG;407_408ins258 | |
| 8 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | NM_001282987.2 | 67.5% | (many diffs) | |
| 9 | human | 317749 | DHRS4L2 | dehydrogenase/reductase 4 l... | NM_001193635.1 | 58.8% | (many diffs) | |
| 10 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | NM_001282991.2 | 56.7% | 9delG;305_306ins360 | |
| 11 | human | 10901 | DHRS4 | dehydrogenase/reductase 4 | XM_011536366.2 | 49.4% | (many diffs) | |
| 12 | human | 728635 | DHRS4L1 | dehydrogenase/reductase 4 l... | NM_001277864.2 | 49% | (many diffs) | |
| 13 | human | 317749 | DHRS4L2 | dehydrogenase/reductase 4 l... | NM_001193636.1 | 46.4% | (many diffs) | |
| 14 | human | 728635 | DHRS4L1 | dehydrogenase/reductase 4 l... | NR_102693.2 | 45.3% | (many diffs) | |
| 15 | human | 728635 | DHRS4L1 | dehydrogenase/reductase 4 l... | NR_102692.2 | 41.8% | (many diffs) | |
| 16 | human | 728635 | DHRS4L1 | dehydrogenase/reductase 4 l... | NR_102689.2 | 40.3% | (many diffs) | |
| 17 | human | 317749 | DHRS4L2 | dehydrogenase/reductase 4 l... | NM_001193637.1 | 31.6% | (many diffs) | |
| 18 | human | 728635 | DHRS4L1 | dehydrogenase/reductase 4 l... | NR_102691.2 | 31.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 84
- ORF length:
- 18
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca caagcggggc tgctaggcct ctgtgcccgg gcttggaatt cggtgcggat 121 ggccagctcc gggatgaccc gccgggaccc gctcgcaaat aaggtggccc tggtaacggc 181 ctccaccgac gggatcggct tcgccatcgc ccggcgtttg gcccaggacg gggcccatgt 241 ggtcgtcagc agccggaagc agcagaatgt ggaccaggcg gtggccacgc tgcaggggga 301 ggggctgagc gtgacgggca ccgtgtgcca tgtggggaag gcggaggacc gggagcggct 361 ggtggccacg gctgtgaagc ttcatggagg tatcgatatc ctagtctcca atgctgctgt 421 caaccctttc tttggaagca taatggatgt cactgaggag gtgtgggaca agactctgga 481 cattaatgtg aaggccccag ccctgatgac aaaggcagtg gtgccagaaa tggagaaacg 541 aggaggcggc tcagtggtga tcgtgtcttc catagcagcc ttcagtccat ctcctggctt 601 cagtccttac aatgtcagta aaacagcctt gctgggcctg accaagaccc tggccataga 661 gctggcccca aggaacatta gggtgaactg cctagcacct ggacttatca agactagctt 721 cagcaggatg ctctggatgg acaaggaaaa agaggaaagc atgaaagaaa ccctgcggat 781 aagaaggtta ggcgagccag aggattgtgc tggcatcgtg tctttcctgt gctctgaaga 841 tgccagctac atcactgggg aaacagtggt ggtgggtgga ggaaccccgt cccgcctctg 901 cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc tcggtctcga 961 ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa tcaacctctg 1021 gatta