Construct: ORF ccsbBroad304_14076
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF016252.1_s304c1, BRDN0000401897
- Derived from:
- ccsbBroadEn_14076
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TMEM131L (23240)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14076
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 23240 | TMEM131L | transmembrane 131 like | NM_015196.3 | 90.7% | 90.7% | 1_444del;2984G>A |
| 2 | human | 23240 | TMEM131L | transmembrane 131 like | NM_001131007.2 | 90.7% | 90.6% | 1_444del;1059_1061delAGC;2987G>A |
| 3 | human | 23240 | TMEM131L | transmembrane 131 like | XM_017007926.1 | 90.7% | 90.6% | 1_444del;2984G>A;3854_3855insAGA |
| 4 | human | 23240 | TMEM131L | transmembrane 131 like | XM_017007925.1 | 90.6% | 90.6% | (many diffs) |
| 5 | human | 23240 | TMEM131L | transmembrane 131 like | XM_024453957.1 | 89.7% | 89.6% | 1_444del;2984G>A;4202_4258del |
| 6 | human | 23240 | TMEM131L | transmembrane 131 like | XM_024453956.1 | 89.6% | 89.6% | (many diffs) |
| 7 | human | 23240 | TMEM131L | transmembrane 131 like | XM_011531781.1 | 89.1% | 89.1% | 1_531del;3071G>A |
| 8 | human | 23240 | TMEM131L | transmembrane 131 like | XM_011531780.1 | 89.1% | 89% | 1_531del;1146_1148delAGC;3074G>A |
| 9 | human | 23240 | TMEM131L | transmembrane 131 like | XM_011531783.1 | 83.9% | 83.9% | (many diffs) |
| 10 | human | 23240 | TMEM131L | transmembrane 131 like | XM_024453958.1 | 65.1% | 65.1% | 0_1ins1488;1052G>A;2270_2326del |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 4452
- ORF length:
- 4383
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggggaaaact tccttcagaa ttattttctt acctactgaa gaaggaagca 121 ttgaaagttc cttatttatt aatacctctt cgtatggagt cctttcctat catgtatctg 181 gaattggcac tcgtagaatc tctacagaag ggtctgcaaa gcagctacca aatgcttatt 241 ttctgcttcc aaaggtccag agcattcagc tgtctcaaat gcaggcagaa accactaata 301 ctagcctctt gcaggtgcaa ctggaatgca gtttacataa taaagtgtgt cagcaattaa 361 agggttgtta tctggaatct gatgatgttt tgcgtctaca aatgagcata atggtaacaa 421 tggaaaactt ttcaaaagaa tttgaagaaa acacacaaca tttgttagat catctctcta 481 ttgtttacgt agctacagat gaatctgaga cctcagatga ttcagcagta aatatgtata 541 tattacattc aggaaacagc cttatatgga tacaggatat acgccatttc tcacagagag 601 atgctctgtc tctgcagttt gaaccagtac tactacctac ttctacaaca aactttacaa 661 aaattgcttc ttttacctgc aaagctacat catgtgacag tggaattata gaagatgtga 721 agaaaacaac acacactcca acactaaaag catgcctctt ctcttctgtg gctcaggggt 781 attttagaat ggactcttct gcaacccagt ttcacataga gactcatgag aacacatcag 841 gactttggtc aatatggtac cgcaaccatt ttgaccgtag tgttgtatta aatgatgtgt 901 ttctttccaa ggagaccaag cacatgttaa agattctgaa tttcactggc cctttatttc 961 tacctccagg ctgttggaat atattttctt tgaaacttgc tgttaaagac attgccataa 1021 atctattcac caatgtattt ttgactacaa acataggtgc catttttgca atacctctac 1081 agatttattc agcaccaacc aaggaaggga gtctgggttt tgaagtgata gcacattgtg 1141 gcatgcatta tttcatggga aaatcaaaag caggaaatcc taattggaat gggagccttt 1201 ctctggatca atctacctgg aatgtggatt ctgaacttgc aaataaattg tatgaaagat 1261 ggaagaaata taaaaatggt gacgtctgca agaggaatgt tttgggaaca actcgatttg 1321 ctcacttgaa gaaatccaag gagtcagagt cctttgtttt ctttttgcct cgtttgatcg 1381 cagagcctgg cctcatgtta aacttcagcg caactgccct taggagcagg atgatcaagt 1441 actttgtggt gcagaacccg tcctcttggc cggtctcctt gcagctcctg cctctctcct 1501 tgtaccctaa acccgaagcc ctagtgcacc tgctccacag atggtttggc actgatatgc 1561 agatgattaa tttcacaact ggtgaattcc agctcaccga agcttgccct tacctgggta 1621 cgcattctga ggaatccagg tttggcatcc tccacttaca tctgcagcct ttggaaatga 1681 aaagggttgg cgtagttttc acacctgctg actatggaaa agttacctca ctcatactaa 1741 tccggaataa cttgactgtt attgacatga ttggcgtgga aggatttgga gcaagagagt 1801 tattaaaagt gggtggaaga cttcctggtg caggaggctc actccgattt aaggtgcccg 1861 agtccacgct gatggactgc cgtagacaac tgaaagacag taagcaaatt ttatctatta 1921 caaagaactt taaagttgag aatattggac ctcttcctat aactgtttcg tctctgaaaa 1981 ttaatgggta taactgccaa ggttatggat tcgaggtgct ggattgtcat cagttttccc 2041 tggacccaaa cacatcccgc gatatcagca ttgtgttcac tccagacttt acctcctcct 2101 gggtaattcg ggacctaagt cttgtaaccg cagcggacct agaatttcgc ttcactctca 2161 atgtgactct ccctcatcac ctgttgccct tgtgtgcaga cgtggttcca ggacccagct 2221 gggaggagtc attttggagg ctcacggtct tctttgtcag tttgtccctg ttgggtgtga 2281 ttttaatagc cttccaacaa gcacagtaca ttctcatgga attcatgaaa acaagacaga 2341 ggcaaaatgc tagctcctct tcacagcaaa acaatggtcc tatggatgta atcagccccc 2401 attcttacaa aagcaattgc aagaactttc tcgatacata tggcccctct gataaaggca 2461 gggggaagaa ctgccttcca gtgaacactc cccaaagcag gatccagaat gctgcaaaga 2521 ggagcccagc cacctatggt cattctcaga agaagcacaa atgctcagtg tattacagta 2581 aacacaaaac cagcacagct gcggccaaca gcaccagcac gactactgag gaaaaacaga 2641 cttcacccct gggcagctca ctgcctgctg ctaaagagga catttgcact gatgccatgc 2701 gtgagaactg gatcagcctc agatatgcaa gtggcataaa tgtcaacctg cagaagaatt 2761 taacccttcc caaaaactta ctgaataaag aagaaaacac actgaaaaac acaattgttt 2821 tcagtaatcc ttcttcagaa tgtagtatga aggagggaat acagacatgt atgtttccta 2881 aggaaactga cattaaaact tcagagaaca cagccgagtt caaggaacgg gagctctgtc 2941 cactgaagac ctccaagaaa ctacctgaaa accatttacc aagaaactca cctcagtacc 3001 accagccaga cttgccagaa atttccagga aaaataatgg gaataaccag caagtacctg 3061 tcaagaatga agtagatcat tgtgaaaatt tgaagaaggt ggacacaaag ccttcttcag 3121 aaaagaagat tcacaaaaca tctagagaag acatgttttc tgagaaacag gacatacctt 3181 tcgtagagca agaagatcct tataggaaga aaaagcttca ggagaaaaga gaaggaaatt 3241 tacaaaattt aaattggagt aaaagtcgaa catgtagaaa gaacaagaaa aggggtgttg 3301 ctccagtctc aaggcctcct gaacagagtg atctaaagct tgtgtgcagt gactttgaga 3361 ggtctgagct gagcagtgac atcaatgtaa gaagctggtg tatacaggaa agcactaggg 3421 aggtttgtaa agcagatgcc gaaattgcaa gcagtttacc tgctgcccag agagaggcag 3481 aaggttacta ccagaagcct gagaagaaat gtgtggacaa gttctgctcc gattccagct 3541 ctgactgtgg gagctcctct ggcagcgtgc gtgccagccg gggcagctgg gggagctgga 3601 gcagcaccag cagctccgac ggggataaga agcccatggt ggacgcccag cacttcctgc 3661 cggccggaga cagtgtttca caaaatgatt ttccttctga agctcccatc tccttgaatc 3721 tttctcataa catctgcaat cccatgaccg tgaatagtct cccacaatac gcagagcctt 3781 cctgtcccag ccttcctgcc gggcccacag gtgttgaaga agataaaggt ctttactcac 3841 ctggagacct gtggcccact ccgccagtgt gtgtgacaag cagcttaaac tgcaccctgg 3901 agaacggcgt gccttgtgtg attcaggagt cggccccggt tcataatagt ttcattgatt 3961 ggagtgcaac atgcgaaggc cagttttcca gcgcatactg tccattggaa ttgaacgatt 4021 acaatgcctt tccagaagaa aacatgaact atgccaatgg cttcccctgt cctgcagatg 4081 ttcagacaga ctttattgat cacaactctc agtctacctg gaacacccca cccaacatgc 4141 ctgctgcctg gggacatgcc agtttcatca gctctccgcc ctacctcaca agcacccgaa 4201 gcttgtctcc aatgtctgga ctttttggtt ccatctgggc cccgcaaagc gatgtgtatg 4261 aaaattgctg ccccatcaac cccaccacgg aacattcgac ccacatggaa aaccaagcgg 4321 tcgtgtgcaa ggaatactac ccggggttca acccgtttcg cgcctatatg aacctggaca 4381 tatggactac cacagcgaat aggaatgcaa atttcccact gtctagagac tcgagttact 4441 gtgggaatgt gttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc 4501 tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga 4561 caatcaacct ctggatta