Construct: ORF ccsbBroad304_14076

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016252.1_s304c1, BRDN0000401897
Derived from:
ccsbBroadEn_14076
DNA Barcode:
None
Epitope Tag:
V5
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
TMEM131L (23240)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14076
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 23240 TMEM131L transmembrane 131 like NM_015196.3 90.7% 90.7% 1_444del;2984G>A
2 human 23240 TMEM131L transmembrane 131 like NM_001131007.2 90.7% 90.6% 1_444del;1059_1061delAGC;2987G>A
3 human 23240 TMEM131L transmembrane 131 like XM_017007926.1 90.7% 90.6% 1_444del;2984G>A;3854_3855insAGA
4 human 23240 TMEM131L transmembrane 131 like XM_017007925.1 90.6% 90.6% (many diffs)
5 human 23240 TMEM131L transmembrane 131 like XM_024453957.1 89.7% 89.6% 1_444del;2984G>A;4202_4258del
6 human 23240 TMEM131L transmembrane 131 like XM_024453956.1 89.6% 89.6% (many diffs)
7 human 23240 TMEM131L transmembrane 131 like XM_011531781.1 89.1% 89.1% 1_531del;3071G>A
8 human 23240 TMEM131L transmembrane 131 like XM_011531780.1 89.1% 89% 1_531del;1146_1148delAGC;3074G>A
9 human 23240 TMEM131L transmembrane 131 like XM_011531783.1 83.9% 83.9% (many diffs)
10 human 23240 TMEM131L transmembrane 131 like XM_024453958.1 65.1% 65.1% 0_1ins1488;1052G>A;2270_2326del
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
69
ORF end:
4452
ORF length:
4383
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcaccat ggggaaaact tccttcagaa ttattttctt acctactgaa gaaggaagca
121ttgaaagttc cttatttatt aatacctctt cgtatggagt cctttcctat catgtatctg
181gaattggcac tcgtagaatc tctacagaag ggtctgcaaa gcagctacca aatgcttatt
241ttctgcttcc aaaggtccag agcattcagc tgtctcaaat gcaggcagaa accactaata
301ctagcctctt gcaggtgcaa ctggaatgca gtttacataa taaagtgtgt cagcaattaa
361agggttgtta tctggaatct gatgatgttt tgcgtctaca aatgagcata atggtaacaa
421tggaaaactt ttcaaaagaa tttgaagaaa acacacaaca tttgttagat catctctcta
481ttgtttacgt agctacagat gaatctgaga cctcagatga ttcagcagta aatatgtata
541tattacattc aggaaacagc cttatatgga tacaggatat acgccatttc tcacagagag
601atgctctgtc tctgcagttt gaaccagtac tactacctac ttctacaaca aactttacaa
661aaattgcttc ttttacctgc aaagctacat catgtgacag tggaattata gaagatgtga
721agaaaacaac acacactcca acactaaaag catgcctctt ctcttctgtg gctcaggggt
781attttagaat ggactcttct gcaacccagt ttcacataga gactcatgag aacacatcag
841gactttggtc aatatggtac cgcaaccatt ttgaccgtag tgttgtatta aatgatgtgt
901ttctttccaa ggagaccaag cacatgttaa agattctgaa tttcactggc cctttatttc
961tacctccagg ctgttggaat atattttctt tgaaacttgc tgttaaagac attgccataa
1021atctattcac caatgtattt ttgactacaa acataggtgc catttttgca atacctctac
1081agatttattc agcaccaacc aaggaaggga gtctgggttt tgaagtgata gcacattgtg
1141gcatgcatta tttcatggga aaatcaaaag caggaaatcc taattggaat gggagccttt
1201ctctggatca atctacctgg aatgtggatt ctgaacttgc aaataaattg tatgaaagat
1261ggaagaaata taaaaatggt gacgtctgca agaggaatgt tttgggaaca actcgatttg
1321ctcacttgaa gaaatccaag gagtcagagt cctttgtttt ctttttgcct cgtttgatcg
1381cagagcctgg cctcatgtta aacttcagcg caactgccct taggagcagg atgatcaagt
1441actttgtggt gcagaacccg tcctcttggc cggtctcctt gcagctcctg cctctctcct
1501tgtaccctaa acccgaagcc ctagtgcacc tgctccacag atggtttggc actgatatgc
1561agatgattaa tttcacaact ggtgaattcc agctcaccga agcttgccct tacctgggta
1621cgcattctga ggaatccagg tttggcatcc tccacttaca tctgcagcct ttggaaatga
1681aaagggttgg cgtagttttc acacctgctg actatggaaa agttacctca ctcatactaa
1741tccggaataa cttgactgtt attgacatga ttggcgtgga aggatttgga gcaagagagt
1801tattaaaagt gggtggaaga cttcctggtg caggaggctc actccgattt aaggtgcccg
1861agtccacgct gatggactgc cgtagacaac tgaaagacag taagcaaatt ttatctatta
1921caaagaactt taaagttgag aatattggac ctcttcctat aactgtttcg tctctgaaaa
1981ttaatgggta taactgccaa ggttatggat tcgaggtgct ggattgtcat cagttttccc
2041tggacccaaa cacatcccgc gatatcagca ttgtgttcac tccagacttt acctcctcct
2101gggtaattcg ggacctaagt cttgtaaccg cagcggacct agaatttcgc ttcactctca
2161atgtgactct ccctcatcac ctgttgccct tgtgtgcaga cgtggttcca ggacccagct
2221gggaggagtc attttggagg ctcacggtct tctttgtcag tttgtccctg ttgggtgtga
2281ttttaatagc cttccaacaa gcacagtaca ttctcatgga attcatgaaa acaagacaga
2341ggcaaaatgc tagctcctct tcacagcaaa acaatggtcc tatggatgta atcagccccc
2401attcttacaa aagcaattgc aagaactttc tcgatacata tggcccctct gataaaggca
2461gggggaagaa ctgccttcca gtgaacactc cccaaagcag gatccagaat gctgcaaaga
2521ggagcccagc cacctatggt cattctcaga agaagcacaa atgctcagtg tattacagta
2581aacacaaaac cagcacagct gcggccaaca gcaccagcac gactactgag gaaaaacaga
2641cttcacccct gggcagctca ctgcctgctg ctaaagagga catttgcact gatgccatgc
2701gtgagaactg gatcagcctc agatatgcaa gtggcataaa tgtcaacctg cagaagaatt
2761taacccttcc caaaaactta ctgaataaag aagaaaacac actgaaaaac acaattgttt
2821tcagtaatcc ttcttcagaa tgtagtatga aggagggaat acagacatgt atgtttccta
2881aggaaactga cattaaaact tcagagaaca cagccgagtt caaggaacgg gagctctgtc
2941cactgaagac ctccaagaaa ctacctgaaa accatttacc aagaaactca cctcagtacc
3001accagccaga cttgccagaa atttccagga aaaataatgg gaataaccag caagtacctg
3061tcaagaatga agtagatcat tgtgaaaatt tgaagaaggt ggacacaaag ccttcttcag
3121aaaagaagat tcacaaaaca tctagagaag acatgttttc tgagaaacag gacatacctt
3181tcgtagagca agaagatcct tataggaaga aaaagcttca ggagaaaaga gaaggaaatt
3241tacaaaattt aaattggagt aaaagtcgaa catgtagaaa gaacaagaaa aggggtgttg
3301ctccagtctc aaggcctcct gaacagagtg atctaaagct tgtgtgcagt gactttgaga
3361ggtctgagct gagcagtgac atcaatgtaa gaagctggtg tatacaggaa agcactaggg
3421aggtttgtaa agcagatgcc gaaattgcaa gcagtttacc tgctgcccag agagaggcag
3481aaggttacta ccagaagcct gagaagaaat gtgtggacaa gttctgctcc gattccagct
3541ctgactgtgg gagctcctct ggcagcgtgc gtgccagccg gggcagctgg gggagctgga
3601gcagcaccag cagctccgac ggggataaga agcccatggt ggacgcccag cacttcctgc
3661cggccggaga cagtgtttca caaaatgatt ttccttctga agctcccatc tccttgaatc
3721tttctcataa catctgcaat cccatgaccg tgaatagtct cccacaatac gcagagcctt
3781cctgtcccag ccttcctgcc gggcccacag gtgttgaaga agataaaggt ctttactcac
3841ctggagacct gtggcccact ccgccagtgt gtgtgacaag cagcttaaac tgcaccctgg
3901agaacggcgt gccttgtgtg attcaggagt cggccccggt tcataatagt ttcattgatt
3961ggagtgcaac atgcgaaggc cagttttcca gcgcatactg tccattggaa ttgaacgatt
4021acaatgcctt tccagaagaa aacatgaact atgccaatgg cttcccctgt cctgcagatg
4081ttcagacaga ctttattgat cacaactctc agtctacctg gaacacccca cccaacatgc
4141ctgctgcctg gggacatgcc agtttcatca gctctccgcc ctacctcaca agcacccgaa
4201gcttgtctcc aatgtctgga ctttttggtt ccatctgggc cccgcaaagc gatgtgtatg
4261aaaattgctg ccccatcaac cccaccacgg aacattcgac ccacatggaa aaccaagcgg
4321tcgtgtgcaa ggaatactac ccggggttca acccgtttcg cgcctatatg aacctggaca
4381tatggactac cacagcgaat aggaatgcaa atttcccact gtctagagac tcgagttact
4441gtgggaatgt gttgccaact ttcttgtaca aagtggttgg taagcctatc cctaaccctc
4501tcctcggtct cgattctacg tagtaatgag ctagcgctaa ccggtggcgc gttaagtcga
4561caatcaacct ctggatta

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