Construct: ORF ccsbBroad304_14096
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF008968.1_s304c1, BRDN0000400078
- Derived from:
- ccsbBroadEn_14096
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DCAF13 (25879)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14096
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25879 | DCAF13 | DDB1 and CUL4 associated fa... | NM_015420.7 | 99.8% | 99.5% | 256G>T;1331T>A |
| 2 | human | 100132724 | DCAF13P3 | DDB1 and CUL4 associated fa... | NR_027642.1 | 51.1% | (many diffs) | |
| 3 | mouse | 223499 | Dcaf13 | DDB1 and CUL4 associated fa... | NM_198606.2 | 89.1% | 92.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 1401
- ORF length:
- 1335
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgaa ggtgaagatg ctgagccgga atccggacaa ttatgtccgc gaaaccaagt 121 tggacttaca gagagttcca agaaactatg atcctgcttt acatcctttt gaggtcccac 181 gagaatatat aagagcttta aatgctacca aactggaacg agtatttgca aaaccattcc 241 ttgcttcgct ggatggtcac cgtgatggag tcaattgctt ggcaaagcat ccagagaagc 301 tggctactgt cctttctggg tcgtgtgatg gagaggttag aatttggaat ctaactcagc 361 ggaattgtat ccgtacaata caagcacatg aaggctttgt acgaggaata tgtactcgct 421 tttgtgggac ttcttttttc actgttggtg atgacaaaac tgtgaagcag tggaaaatgg 481 atgggccagg ctatggagac gaggaagagc cattacatac aatattagga aagacagtgt 541 atactgggat tgatcatcac tggaaagaag ctgtttttgc cacatgtgga cagcaagtag 601 acatttggga tgaacaaaga actaatccta tatgttcaat gacctgggga tttgacagta 661 taagtagtgt taaatttaac ccaattgaga catttctctt gggaagttgt gcatctgaca 721 ggaatatagt actgtacgat atgaggcaag ctactccttt gaaaaaggtt atcttagata 781 tgagaacaaa tacaatctgt tggaacccta tggaagcttt catttttaca gcagcaaatg 841 aagattataa cttatatact tttgatatgc gtgcactgga cactcctgta atggtccata 901 tggatcatgt atctgcagtg cttgatgtgg attactctcc cactgggaag gagtttgtgt 961 ctgctagttt cgataaatct attcgaatct ttcctgtaga caaaagtcga agcagggagg 1021 tatatcatac aaagagaatg caacatgtta tctgtgtaaa atggacttct gacagcaagt 1081 atattatgtg tggatctgat gaaatgaaca ttcgcctgtg gaaagctaat gcttctgaaa 1141 aattgggtgt gcttacatca cgagaaaaag cagccaagga ttataaccag aaattgaagg 1201 agaaatttca gcattatcct catataaaac gtatagctcg tcatcgacat ctaccaaaat 1261 ctatctatag ccagattcag gaacagcgca tcatgaaaga agctcgtcga cgaaaggaag 1321 tgaatcgtat taaacacagc aagcctggat ctgtgccact tgtgtcagag aagaagaaac 1381 acgtagtggc agttgaaaaa tacccaactt tcttgtacaa agtggttggt aagcctatcc 1441 ctaaccctct cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg 1501 ttaagtcgac aatcaacctc tggatta