Construct: ORF ccsbBroad304_14099
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF013211.1_s304c1, BRDN0000402610
- Derived from:
- ccsbBroadEn_14099
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- NECTIN3 (25945)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14099
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 25945 | NECTIN3 | nectin cell adhesion molecu... | XM_017006127.2 | 29.8% | 29.6% | 1_708del;721T>C |
| 2 | human | 25945 | NECTIN3 | nectin cell adhesion molecu... | XM_017006125.1 | 19.1% | 19% | 1_1275del;1288T>C |
| 3 | human | 25945 | NECTIN3 | nectin cell adhesion molecu... | XM_017006124.1 | 18.8% | 18.7% | 1_1299del;1312T>C |
| 4 | human | 25945 | NECTIN3 | nectin cell adhesion molecu... | NM_015480.3 | 18.3% | 18.2% | 1_1344del;1357T>C |
| 5 | human | 25945 | NECTIN3 | nectin cell adhesion molecu... | XM_011512663.1 | 17.3% | 17.2% | 1_1437del;1450T>C |
| 6 | human | 25945 | NECTIN3 | nectin cell adhesion molecu... | XR_924122.2 | 12.7% | 1_1667del;1680T>C;1971_2368del | |
| 7 | human | 25945 | NECTIN3 | nectin cell adhesion molecu... | XR_002959508.1 | 10.8% | 1_1625del;1638T>C;1929_2772del | |
| 8 | mouse | 58998 | Nectin3 | nectin cell adhesion molecu... | NM_021495.4 | 16.3% | 17.1% | (many diffs) |
| 9 | mouse | 58998 | Nectin3 | nectin cell adhesion molecu... | XR_384583.3 | 7.7% | (many diffs) | |
| 10 | mouse | 58998 | Nectin3 | nectin cell adhesion molecu... | XR_001781847.1 | 2.7% | (many diffs) | |
| 11 | mouse | 58998 | Nectin3 | nectin cell adhesion molecu... | XR_001781848.1 | 2.7% | (many diffs) | |
| 12 | mouse | 58998 | Nectin3 | nectin cell adhesion molecu... | XR_001781846.1 | 2.6% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 369
- ORF length:
- 303
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatgca aaaagaacca caaatagatg ttcttcaaca agatgagctt gattcttacc 121 cagacagtgt aaaaaaagaa aacaaaaatc cagtgaacaa tctaatacgt aaagactatt 181 tagaagagcc tgaaaaaact cagtggaaca atgtagaaaa tctcaatagg tttgaaagac 241 caatggatta ttatgaagat ctaaaaatgg gaatgaagtt tgtcagtgat gaacattatg 301 atgaaaacga agatgactta gtttcacatg tagatggttc cgtaatttcc aggagggagt 361 ggtatgttta cccaactttc ttgtacaaag tggttggtaa gcctatccct aaccctctcc 421 tcggtctcga ttctacgtag taatgagcta gcgctaaccg gtggcgcgtt aagtcgacaa 481 tcaacctctg gatta