Transcript: Human XM_017006127.2

PREDICTED: Homo sapiens nectin cell adhesion molecule 3 (NECTIN3), transcript variant X8, mRNA.

Source:
NCBI, updated 2019-09-08
Taxon:
Homo sapiens (human)
Gene:
NECTIN3 (25945)
Length:
5318
CDS:
971..1984

Additional Resources:

NCBI RefSeq record:
XM_017006127.2
NBCI Gene record:
NECTIN3 (25945)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human XM_017006127.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000419546 ACCACCCTTCAGCCTACAATT pLKO_005 1418 CDS 100% 13.200 18.480 N NECTIN3 n/a
2 TRCN0000063347 GTGCCTTAGCTGGACCAATTA pLKO.1 495 5UTR 100% 13.200 18.480 N NECTIN3 n/a
3 TRCN0000431295 ATCCGATACTCTTTCATATTA pLKO_005 1103 CDS 100% 15.000 10.500 N NECTIN3 n/a
4 TRCN0000431857 ATCAGCCAGTACAAGCTATTT pLKO_005 1016 CDS 100% 13.200 9.240 N NECTIN3 n/a
5 TRCN0000063343 GCGAATTACTTGTGTTGTAAA pLKO.1 1060 CDS 100% 13.200 9.240 N NECTIN3 n/a
6 TRCN0000426482 GTGAACAATCTAATACGTAAA pLKO_005 1766 CDS 100% 10.800 7.560 N NECTIN3 n/a
7 TRCN0000063345 GCCCTTCCCATTGTCAACTTT pLKO.1 1495 CDS 100% 5.625 3.938 N NECTIN3 n/a
8 TRCN0000063344 GCCACGATCATTGCTAGTGTA pLKO.1 1541 CDS 100% 4.950 3.465 N NECTIN3 n/a
9 TRCN0000063346 CCATCCATTGACTTTCAATTA pLKO.1 1309 CDS 100% 13.200 7.920 N NECTIN3 n/a
10 TRCN0000341001 TCACTTAATGATGCAACAATT pLKO_005 713 5UTR 100% 13.200 7.920 N Nectin3 n/a
11 TRCN0000109443 CGTGGAGACTACTTTGCCAAA pLKO.1 1637 CDS 100% 4.050 2.835 N Nectin3 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript XM_017006127.2, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14099 pDONR223 100% 29.8% 29.6% None 1_708del;721T>C n/a
2 ccsbBroad304_14099 pLX_304 0% 29.8% 29.6% V5 1_708del;721T>C n/a
3 TRCN0000479111 TCGATAAGTATCGTTCATATCGAC pLX_317 100% 29.8% 29.6% V5 1_708del;721T>C n/a
4 ccsbBroadEn_11789 pDONR223 100% 27.9% 26.2% None (many diffs) n/a
5 ccsbBroad304_11789 pLX_304 0% 27.9% 26.2% V5 (many diffs) n/a
6 TRCN0000480602 CTCGCGGCTGGCTCAATGGGTCCT pLX_317 36% 27.9% 26.2% V5 (many diffs) n/a
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