Construct: ORF ccsbBroad304_14145

Construct Description:

• Construct Type: ORF
• Other Identifiers: ORF009491.1_s304c1, BRDN0000403183
• Derived from: ccsbBroadEn_14145
• DNA Barcode: None
• Epitope Tag: V5
• Notes: No stop codon in insert

Originally Annotated References:

• Gene: ANKMY1 (51281)

Vector Information:

• Vector Backbone: pLX_304
• Pol II Cassette 1: PGK-BlastR
• Pol II Cassette 2: CMV-ccsbBroad304_14145
• Selection Marker: BlastR
• Visible Reporter: n/a
• Epitope Tag: V5

Current transcripts matched by this ORF:

	Taxon	Gene	Symbol	Description	Transcript	Nuc. Match %	Prot. Match %	Match Diffs
1	human	51281	ANKMY1	ankyrin repeat and MYND dom...	NM_001354026.1	66.5%	66.5%	0_1ins267;876_877ins174
2	human	51281	ANKMY1	ankyrin repeat and MYND dom...	NM_017844.4	61%	60.8%	(many diffs)
3	human	51281	ANKMY1	ankyrin repeat and MYND dom...	NM_001282780.2	60.8%	60.5%	(many diffs)
4	human	51281	ANKMY1	ankyrin repeat and MYND dom...	NM_001308375.2	51.9%	51.7%	(many diffs)
5	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_017004285.2	48.6%	48.3%	(many diffs)
6	human	51281	ANKMY1	ankyrin repeat and MYND dom...	NR_148697.2	46.5%		(many diffs)
7	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_024452955.1	43.3%	43.2%	(many diffs)
8	human	51281	ANKMY1	ankyrin repeat and MYND dom...	NM_001282771.3	42.5%	42.3%	(many diffs)
9	human	51281	ANKMY1	ankyrin repeat and MYND dom...	NM_001354023.2	42.5%	42.3%	(many diffs)
10	human	51281	ANKMY1	ankyrin repeat and MYND dom...	NM_001282781.2	42.5%	42.2%	(many diffs)
11	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_017004289.1	39%	38.9%	(many diffs)
12	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_017004288.1	38.8%	38.7%	0_1ins267;1048delC;1051_2436delinsT
13	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_017004275.2	38.7%	38.5%	(many diffs)
14	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_017004276.1	38.7%	38.5%	(many diffs)
15	human	51281	ANKMY1	ankyrin repeat and MYND dom...	NR_148696.2	38.7%		(many diffs)
16	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_017004287.1	38.6%	38.5%	(many diffs)
17	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_017004290.1	36.1%	30%	(many diffs)
18	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_024452952.1	35.7%	34.8%	(many diffs)
19	human	51281	ANKMY1	ankyrin repeat and MYND dom...	NM_001354024.2	35.5%	35.5%	(many diffs)
20	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_024452954.1	34.5%	34.4%	(many diffs)
21	human	51281	ANKMY1	ankyrin repeat and MYND dom...	NM_016552.5	33.9%	33.8%	(many diffs)
22	human	51281	ANKMY1	ankyrin repeat and MYND dom...	NM_001354025.2	33%	32.9%	0_1ins570;745delC;748_1689delinsT
23	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_024452958.1	33%	32.9%	0_1ins570;745delC;748_1689delinsT
24	human	51281	ANKMY1	ankyrin repeat and MYND dom...	NR_148698.1	31.6%		(many diffs)
25	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_017004281.1	30.9%	30.8%	(many diffs)
26	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_017004282.1	30.9%	30.8%	(many diffs)
27	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_024452953.1	30.9%	30.8%	(many diffs)
28	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_024452956.1	27.5%	27.5%	0_1ins570;745delC;748_2133delinsT
29	human	51281	ANKMY1	ankyrin repeat and MYND dom...	XM_024452957.1	27.5%	27.5%	0_1ins570;745delC;748_2133delinsT

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:

66

ORF end:

1383

ORF length:

1317

Sequence:

1	ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61	ttggcatgga angggcccan gcctccctta gcttagagga cgaagtctct ggggctggca
121	gccgccaacg cccgctagag gggaagggcg gcgagacccc tgctgccgag gagccggggt
181	ccctgaagaa ctacgctgtc ttcgccacaa gggatgtttc agcagcccct gagaaggaag
241	aggaggaagc tgagggcccg ctgagggcgc aggacctgag ggagtcctac atccagctcg
301	tccagggtgt gcaggagtgg caggatggtt gcatgtacca gggggagttt gggttgaaca
361	tgaagcttgg atatggcaaa ttctcttggc ccacaggcga gtcataccat gggcagtttt
421	accgggacca ctgccatggc ctgggtacct acatgtggcc agatggctcc agtttcacgg
481	gcacatttta cctcagccac cgagaaggct acggcaccat gtacatgaag acacggcttt
541	tccagactca ctgccacaac gacattgtca accttctcct ggactgtggg gccgacgtga
601	acaagtgctc agatgagggt ctcacggcac tcagcatgtg tttcctcctc cactaccccg
661	cccagtcctt caagcccaat gttgctgaac ggaccatacc tgagccccag gaacctccaa
721	aattcccagt tgttccaatc ctttcatcat catttatgga cacaaacctg gagtctctgt
781	actatgaggt gaacgtgcct tcccagggta gctatgagct gaggccaccg ccagcaccac
841	tgctcctgcc acgcgtctca ggcagccacg agggcggcca cttccaggac accgggcagt
901	gtggggggtc catagaccac aggagcagct ctctgaaggg ggactccccg ttggtgaagg
961	gcagccttgg ccatgtggaa agcgggcttg aggacgtgtt gggaaacaca gaccggggca
1021	gtctgtgcag tgctgagacg aaatttgagt ccaacgtgtg tgtgtgcgac ttctccatcg
1081	agctctcgca ggccatgctg gagagaagcg cccagtccca cagcttgctg aagatggcct
1141	cgccctcacc gtgcaccagc agcttcgaca aagggaccat gcggaggatg gcgctgtcca
1201	tgatcgagcg gaggaagcgc tggcggacca tcaagctgct gctgcgccgg ggcgcggacc
1261	ccaacctgtg ctgcgtgccc atgcaggtcc tgttccttgc tgtgaaggcc ggggacgtgg
1321	atggggtgag gctgctgctg gagcacgggg cgaggaccga catctgcttt ccgccgcagt
1381	gttgcccaac tttcttgtac aaagtggttg gtaagcctat ccctaaccct ctcctcggtc
1441	tcgattctac gtagtaatga gctagcgcta accggtggcg cgttaagtcg acaatcaacc
1501	tctggatta

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