Construct: ORF ccsbBroad304_14193

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006915.1_s304c1, BRDN0000461540
Derived from:
ccsbBroadEn_14193
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
OGFOD1 (55239)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14193
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 55239 OGFOD1 2-oxoglutarate and iron dep... NM_018233.4 99.9% 99% 1611delA
2 human 55239 OGFOD1 2-oxoglutarate and iron dep... NM_001324357.2 99.7% 98.8% 153_154insGAA;1608delA
3 human 55239 OGFOD1 2-oxoglutarate and iron dep... NM_001324363.2 92.9% 92% 786_787ins114;1497delA
4 human 55239 OGFOD1 2-oxoglutarate and iron dep... NM_001324358.1 89.7% 88.9% 0_1ins165;1446delA
5 human 55239 OGFOD1 2-oxoglutarate and iron dep... NM_001324361.1 89.7% 88.9% 0_1ins165;1446delA
6 human 55239 OGFOD1 2-oxoglutarate and iron dep... NM_001324359.1 82% 80.6% (many diffs)
7 human 55239 OGFOD1 2-oxoglutarate and iron dep... NM_001324360.2 82% 80.6% (many diffs)
8 human 55239 OGFOD1 2-oxoglutarate and iron dep... NM_001324362.1 74.1% 73.2% 0_1ins420;1191delA
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
1692
ORF length:
1626
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgaa tgggaagcgg ccagcggagc ccggcccagc ccgggtggga aaaaagggaa
121agaaggaggt gatggcggag ttttcggacg ctgttacgga agaaaccttg aaaaagcagg
181tggctgaggc ctggagccgc aggacgccgt tcagtcacga agtcattgtc atggacatgg
241acccttttct tcactgtgtg atcccaaact tcatccaaag ccaagacttc ttagaagggc
301ttcagaagga actgatgaac ttggacttcc atgagaagta taatgattta tataagttcc
361agcagtctga tgatttgaag aagagaagag agcctcacat ctccacttta aggaaaattc
421tgtttgaaga tttccggtcc tggctttctg atatttctaa aattgacctg gaatcaacca
481ttgacatgtc ctgtgctaaa tatgaattca ctgatgccct gctgtgccat gatgatgagc
541tggaagggcg ccggattgcc ttcatcctgt acctggttcc tccctgggac aggagcatgg
601gtggtaccct ggacctgtac agcattgatg aacactttca gccgaagcag attgtcaagt
661ctcttatccc ttcgtggaac aaactggttt tctttgaagt atctcctgtg tcctttcacc
721aggtgtctga agtgctgtct gaagaaaagt cacgtttgtc tataagtggc tggtttcatg
781gtccatcatt gactcggcct cccaactact ttgaaccccc catacctcgg agccctcaca
841tcccacaaga tcatgagatt ttgtatgatt ggatcaaccc tacttatctg gacatggatt
901accaagttca aattcaagaa gagtttgaag aaagttctga aattctcctg aaggagtttc
961ttaagcctga gaaattcacg aaagtctgtg aggccttgga gcatggacat gtggaatgga
1021gcagccgagg tccccctaac aaaaggtttt atgagaaagc tgaggagagt aagcttcctg
1081agatattgaa ggagtgcatg aagttatttc gctctgaggc actattcttg ctgctctcca
1141acttcacagg cctgaagctt catttcttgg ccccttcgga agaagatgag atgaatgata
1201aaaaagaggc agaaaccact gatatcactg aagaagggac tagccatagt cctcctgagc
1261cagagaataa tcagatggcc atcagcaaca acagccaaca gagcaatgag cagacagacc
1321cagagccaga ggaaaatgaa acaaagaaag aatcaagtgt tcccatgtgc caaggggaac
1381tgaggcattg gaagaccggt cactacactt taattcatga ccatagcaag gctgaatttg
1441ccctagactt aattctgtac tgtggctgtg aaggctggga gccagaatat ggcggtttta
1501cttcttacat tgccaaaggt gaagatgaag agctgctaac agtgaatcca gaaagcaatt
1561ctttggcatt ggtctacaga gacagagaga ctctgaaatt tgtcaagcat attaaccacc
1621gaagcctgga acaaaagaaa accttcccaa acagaacagg tttctgggac ttttcttcat
1681ctattatgaa tgcccaactt tcttgtacaa agtggttggt aagcctatcc ctaaccctct
1741cctcggtctc gattctacgt agtaatgagc tagcgctaac cggtggcgcg ttaagtcgac
1801aatcaacctc tggatta

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