Construct: ORF ccsbBroad304_14319

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF016104.1_s304c1, BRDN0000409846
Derived from:
ccsbBroadEn_14319
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to prior stop codon)
Notes:
Early stop codon detected

Originally Annotated References:

Gene:
RNFT2 (84900)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14319
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 84900 RNFT2 ring finger protein, transm... NM_032814.4 47.5% 35.4% (many diffs)
2 human 84900 RNFT2 ring finger protein, transm... NM_001109903.1 45% 33.6% (many diffs)
3 human 84900 RNFT2 ring finger protein, transm... XR_001748905.2 43% (many diffs)
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Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
708
ORF length:
642
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatgcc cgcgtccagg gaggaaggag ggggccgggg cgaggggggc gcctaccacc
121accgccagcc ccaccaccat ttccaccatg gcggccaccg cgggggctcc ctgctgcagc
181acgtgggtgg ggaccaccgg gggcactcgg aggagggagg cgacgagcag cctgggacgc
241ccgcccccgc cctgtccgag ctgaaggctg tgatctgctg gctccagaaa ggactcccct
301tcatcctgat cctcctggcc aaactgtgct ttcagcataa gctcggcatt gctgtgtgca
361tcgggatggc cagcaccttc gcctatgcca actccacgct tcgagaacag gtctcactga
421aggagaagag gtcagtgctg gtcatcttgt ggatcctggc ctttctggcg gggaacaccc
481tctatgtgct ttatacattc agctccgaga ttgcgccaca gcactccaac ctgggtgaca
541gagtgagtga gactctgtct aaaaaaaaaa gaaagaaaga agggagggag ggaggaagga
601aggaaggaag gaaagaaaga aagctcagct ttggcccaga tgtggttacc ccttggtctc
661ctgtctttat gtctttctcc tcttcctatt ctgtcatctc cctcacttaa gtctcaggcc
721tgtcagcagc tcctgtggac attgccatcc cctctggtac ccaactttct tgtacaaagt
781ggttggtaag cctatcccta accctctcct cggtctcgat tctacgtagt aatgagctag
841cgctaaccgg tggcgcgtta agtcgacaat caacctctgg atta

Download FASTA (ORF) (Full)