Construct: ORF ccsbBroad304_14326
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010537.1_s304c1, BRDN0000408962
- Derived from:
- ccsbBroadEn_14326
- DNA Barcode:
- None
- Epitope Tag:
- V5
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- USP45 (85015)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14326
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 85015 | USP45 | ubiquitin specific peptidas... | NM_001346033.1 | 99.5% | 98.6% | (many diffs) |
| 2 | human | 85015 | USP45 | ubiquitin specific peptidas... | NM_032929.2 | 99.5% | 98.6% | (many diffs) |
| 3 | human | 85015 | USP45 | ubiquitin specific peptidas... | NM_001346030.2 | 64.9% | 64.3% | (many diffs) |
| 4 | human | 85015 | USP45 | ubiquitin specific peptidas... | XM_017011385.2 | 64.4% | 63.8% | (many diffs) |
| 5 | human | 85015 | USP45 | ubiquitin specific peptidas... | XM_017011386.2 | 64.4% | 63.8% | (many diffs) |
| 6 | human | 85015 | USP45 | ubiquitin specific peptidas... | NM_001346026.2 | 39.4% | 38.9% | (many diffs) |
| 7 | human | 85015 | USP45 | ubiquitin specific peptidas... | NM_001346024.2 | 37.5% | 37.1% | (many diffs) |
| 8 | human | 85015 | USP45 | ubiquitin specific peptidas... | NM_001346025.2 | 37.4% | 37% | (many diffs) |
| 9 | human | 85015 | USP45 | ubiquitin specific peptidas... | NM_001080481.2 | 34.9% | 34.5% | (many diffs) |
| 10 | human | 85015 | USP45 | ubiquitin specific peptidas... | NM_001346021.2 | 34.9% | 34.5% | (many diffs) |
| 11 | human | 85015 | USP45 | ubiquitin specific peptidas... | NM_001346022.2 | 34.9% | 34.5% | (many diffs) |
| 12 | human | 85015 | USP45 | ubiquitin specific peptidas... | XM_005267170.4 | 34.9% | 34.5% | (many diffs) |
| 13 | human | 85015 | USP45 | ubiquitin specific peptidas... | NM_001346023.2 | 34.8% | 34.3% | (many diffs) |
| 14 | human | 85015 | USP45 | ubiquitin specific peptidas... | XR_001743700.2 | 16.9% | (many diffs) | |
| 15 | human | 85015 | USP45 | ubiquitin specific peptidas... | NR_144348.2 | 16.6% | (many diffs) | |
| 16 | human | 85015 | USP45 | ubiquitin specific peptidas... | NR_144345.2 | 16.3% | (many diffs) | |
| 17 | human | 85015 | USP45 | ubiquitin specific peptidas... | NR_144347.2 | 16% | (many diffs) | |
| 18 | human | 85015 | USP45 | ubiquitin specific peptidas... | NR_144344.2 | 15.1% | (many diffs) | |
| 19 | human | 85015 | USP45 | ubiquitin specific peptidas... | NR_144346.2 | 14.9% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 65
- ORF end:
- 932
- ORF length:
- 867
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggatgcgg gtgaaagatc caactaaagc tttacctgag aaagccaaaa gaagtaaaag 121 gcctactgta cctcatgatg aagactcttc agatgatatt gttgtaggtt taacttgcca 181 acatgtaagt catgctatca gcgtgaatca tgtacagaga gcaatagctg agaatctgtg 241 gtcagtttgc tcagaatgtt tagaagaaag aagattctat gatgggcagc tagtacttac 301 ttctgatatt tggttgtgcc tcaagtgtgg cttccaggga tgtggtaaaa actcagaaag 361 ccaacattca ttgaagcact ttaagagttc cagaacagag ccccattgta ttataattaa 421 tctgagcaca tggattatat ggtgttatga atgtgatgaa aaattatcaa cgcattgtaa 481 taagaaggtt ttggctcaga tagttgattt tctccagaaa catgcttcta aaacacaaac 541 aagtgcattt tctagaatca tgaaactttg tgaagaaaaa tgtgaaacag atgaaataca 601 gaagggagga aaatgcagaa atttatctgt aagaggaatt acaaatttag gaaatacttg 661 cttttttaat gcagtcatgc agaacttggc acagacttat actcttactg atctgatgaa 721 tgagatcaaa gaaagtagta caaaactcaa gatttttcct tcctcagact ctcagctgga 781 cccattggtg gtggaacttt caaggcctgg accactgacc tcagccttgt tcctgtttct 841 tcacagcatg aaggagactg aaaaaggacc actttctcct aaagttcttt ttaatcagct 901 ttgtcagaag cgggtgcatc tacatttaat atacccaact ttcttgtaca aagtggttgg 961 taagcctatc cctaaccctc tcctcggtct cgattctacg tagtaatgag ctagcgctaa 1021 ccggtggcgc gttaagtcga caatcaacct ctggatta