Construct: ORF ccsbBroad304_14363
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF010767.1_s304c1, BRDN0000410839
- Derived from:
- ccsbBroadEn_14363
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- DNAJC24 (120526)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14363
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 120526 | DNAJC24 | DnaJ heat shock protein fam... | NM_181706.5 | 99.3% | 99.3% | 1_3delATG |
| 2 | human | 120526 | DNAJC24 | DnaJ heat shock protein fam... | XR_002957125.1 | 45.1% | 1_109del;426_549del;678_983del | |
| 3 | human | 120526 | DNAJC24 | DnaJ heat shock protein fam... | XR_001747771.2 | 42.4% | 1_109del;424_821del;911_912ins40 |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 66
- ORF end:
- 510
- ORF length:
- 444
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcatggc ggttgagcag atgccaaaaa aggattggta cagcatcctg ggagcagacc 121 catctgcaaa tatatcagac ctaaaacaaa aatatcaaaa actcatatta atgtatcatc 181 cagataaaca aagtacagat gtaccagcag gaacagtgga ggaatgtgta cagaagttca 241 tcgaaattga tcaagcatgg aaaattctag gaaatgaaga gacaaaaaga gagtatgacc 301 tgcagcggtg tgaagatgat ctaagaaatg taggaccagt agatgctcaa gtatatcttg 361 aagaaatgtc ttggaatgaa ggtgatcact ctttttatct gagttgcaga tgtggtggaa 421 aatacagtgt ttccaaggat gaagcggaag aagttagcct gatttcttgt gatacatgtt 481 cactaattat agaactcctt cattataact cccaactttc ttgtacaaag tggttggtaa 541 gcctatccct aaccctctcc tcggtctcga ttctacgtag taatgagcta gcgctaaccg 601 gtggcgcgtt aagtcgacaa tcaacctctg gatta