Construct: ORF ccsbBroad304_14371
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF007182.1_s304c1, BRDN0000462330
- Derived from:
- ccsbBroadEn_14371
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to prior stop codon)
- Notes:
- Early stop codon detected
Originally Annotated References:
- Gene:
- MYOM3 (127294)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14371
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 127294 | MYOM3 | myomesin 3 | NM_152372.4 | 83.4% | 46.5% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 2211
- ORF length:
- 2142
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat gactctgccg cacagcttgg gaggtgcggg ggacccccgg cccccccagg 121 ccatggaggt tcacaggctg gagcaccggc aggaggagga gcagaaggag gagcggcagc 181 acagcctgcg catgggctcc tctgtgcgga ggcggacctt ccgcagcagc gaagaagagc 241 atgagttcag cgccgcggac tacgccctgg cagcagccct ggctctgacg gcctcctccg 301 agctgtcttg ggaagcccag ctgagacgcc agacctctgc cgtggagctg gaggagcgag 361 ggcagaagcg ggtgggcttc ggcaatgact gggagaggac tgagatcgcc ttcctgcaga 421 cccaccggct gctgcgccag aggcgggact ggaagacgct gaggcggcgg acagaggaga 481 aggtccagga ggccaaggag ctgagggagc tgtgctacgg ccgcgggccc tggttctgga 541 tccctcttcg ctcccacgcc gtctgggagc acaccacggt cctgctgacc tgcactgtcc 601 aggcctcacc accaccccag gtcacctggt acaaaaatga cacacggatt gatccccgcc 661 tctttcgtgc cggaaaatac cgaatcacca acaactacgg gctgctgtcc ctggagatta 721 ggagatgcgc cattgaggac tcagcaactt acactgtgcg agtgaagaac gcccacggcc 781 aggcctcctc cttcgccaaa gtcctcgtcc gcacttacct ggggaaggat gctggcttcg 841 attcagagat cttcaaaaga tcgacgtttg gccccagcgt ggaattcacc tcggtgctga 901 agccagtctt tgctcgtgag aaggaaccct tctccctgtc atgcttgttt tcggaagatg 961 tgttagatgc tgagagcatc cagtggttcc gagatgggag cctactgagg tcctcgagac 1021 gtcggaagat cctctacaca gaccgccagg catccctgaa ggtgtcctgc acctacaagg 1081 aggacgaggg gctctacatg gtccgggtgc cctcgccctt cggaccccgg gaacagagca 1141 cctacgtgct tgtgagagat gccgaggccg agaaccccgg ggccccaggc tccccactga 1201 acgtccgatg cctggatgtg aacagagact gcctcatcct gacttgggcc ccgcccagtg 1261 acacccgggg caaccccatc actgcctaca ccattgagcg gtgccagggc gagtctgggg 1321 aatggatcgc ctgccatgag gcccccggag ggacttgtcg gtgcccaatc caaggcctcg 1381 tcgaaggtca gagctatcgg ttccgggtga gagccatcag cagggtaggc agcagcgtcc 1441 cctccaaggc ctcagagttg gttgtcatgg gtgaccatga tgcagcccgg aggaagacag 1501 agatcccctt tgatctggga aacaagatca ccatcagcac agacgctttt gaagatactg 1561 tgaccatccc ctcaccgcca accaatgtcc atgccagcga gatccgagag gcctatgtgg 1621 ttctggcctg ggaggagccc agcccccggg gcagagcacc actgacgtac tccctggaga 1681 agtcagtcat aggtagtggc acctgggagg ccatcagctc ggaaagccct gtgagatccc 1741 cgagattcgc cgttctggac ctggagaaaa agaagtcgta tgtcttcaga gtgcgagcaa 1801 tgaaccagta tggcctgagc gatccctcgg agcccagcga acccatcgcc ttgcggggcc 1861 cgccagctac cctccctcct ccagctcaag ttcaagcttt cagagacaca cagacctctg 1921 tctccctgac atgggatcct gtgaaagacc cagagctcct gggttattac atctactccc 1981 ggaaggtggg gacatctgag tggcaaacag ttaacaacaa acccatccaa ggcaccaagg 2041 tttacagttc ccgggctgag gacggggaag gagtacgagt tttgtgtcag gtcagtcagc 2101 gaggctgggg taggcgagag ctcagccgcc accgagccca tcagggtcaa gcaggctctg 2161 gctaccccgt ctgccccata tggctttgcc ctcctgaact gcgggaagaa tgaaatggtc 2221 attgggtgga aaccccccaa gcgtcgtgga ggtggcaaga tcctgggcta cttcctggac 2281 cagcatgact cggaagagct ggactggcat gcggtcaatc agcagcccat ccccacccgg 2341 gtctgcaagg tcagtgacct tcatgaaggc cacttctatg agttccgtgc ccgggctgcc 2401 aactgggcag gtgttggcga gctgtcggca cccagcagcc tgtttgagtg caaagagtgg 2461 acaatgcccc agccaggccc cccgtacgat gtacgggcat ccgaggtgcg ggccacatcc 2521 ctggtgctgc agtgggaacc cccactgtat atgggggctg ggcctgtcac aggctatcac 2581 gtcagtttcc aggaggaagg ctctgagcag tggaagccgg tcaccccagg ccccatctct 2641 ggcacccacc tgagggtttc cgacttgcag ccaggaaaga gttacgtgtt ccaggtacag 2701 gccatgaatt cagctggtct ggggcaaccg tccatgccca ctgatcccgt gctcctggag 2761 gacaagccag gtgcccatga gatcgaggtt ggtgtggatg aagagggctt tatctatttg 2821 gcttttgaag cccctgaagc ccccgactcc tcagagtttc agtggtccaa agactacaag 2881 ggcccactgg acccccagag ggtcaagatc gaggataaag ttaacaagtc caaggtcatc 2941 ctgaaagaac ccggcctcga ggatttgggc acctactcag tcatagtcac tgatgccgat 3001 gaagacatct ccgcaagcca cacgctaacc gaggaagagc tggagaagct gaagaagctg 3061 agtcatgaga tcagaaaccc agtgatcaaa ctgatctccg gctggaacat tgacatcctg 3121 gagcgagggg aggtgcggct ttggctggaa gtagaaaagt tatctccagc cgctgagcta 3181 catctaatct tcaacaacaa ggagatcttc agctcgccga accgcaaaat caattttgac 3241 cgagagaagg gcctggtgga agtgatcatc cagaacttgt ctgaggagga caaagggtcg 3301 tacaccgctc aactccaaga tggaaaagcc aaaaaccaga ttaccttgac actggtggat 3361 gacgattttg acaagcttct gaggaaggca gatgctaaga gaagggactg gaagagaaaa 3421 cagggtccct attttgagcg gccgttgcag tggaaggtca cggaggactg ccaagtgcag 3481 ctgacgtgca aggtgaccaa caccaagaag gagactcgct ttcagtggtt cttccagagg 3541 gcagagatgc cagatggtca gtatgaccca gagacgggaa cgggacttct ctgcattgaa 3601 gaggcaagtg tggctccagc ttcagggctc actttctcct ttggctgcaa gccagggagg 3661 gccaagagct ctgggctggg agtcagggca gggccgaggc taggtttgcc aactttcttg 3721 tacaaagtgg ttggtaagcc tatccctaac cctctcctcg gtctcgattc tacgtagtaa 3781 tgagctagcg ctaaccggtg gcgcgttaag tcgacaatca acctctggat ta