Construct: ORF ccsbBroad304_14415
Construct Description:
- Construct Type:
- ORF
- Other Identifiers:
- ORF002067.1_s304c1, BRDN0000460431
- Derived from:
- ccsbBroadEn_14415
- DNA Barcode:
- None
- Epitope Tag:
- V5 (not translated due to frame shift)
- Notes:
- No stop codon in insert
Originally Annotated References:
- Gene:
- TCEAL6 (158931)
Vector Information:
- Vector Backbone:
- pLX_304
- Pol II Cassette 1:
- PGK-BlastR
- Pol II Cassette 2:
- CMV-ccsbBroad304_14415
- Selection Marker:
- BlastR
- Visible Reporter:
- n/a
- Epitope Tag:
- V5
Current transcripts matched by this ORF:
| Taxon | Gene | Symbol | Description | Transcript | Nuc. Match %[?]A simple nucleotide-based global alignment percentage, calculated as follows: total nt. matches ---------------------------------- aligned length (incl. gaps) |
Prot. Match %[?]A simple amino acid-based global alignment percentage, calculated as follows: total aa. matches ---------------------------------- aligned length (incl. gaps) |
Match Diffs[?]This field may contain sequence annotations in HGVS format. For more information about HGVS annotations, please refer to the HGVS Quick Reference Guide. | |
|---|---|---|---|---|---|---|---|---|
| 1 | human | 158931 | TCEAL6 | transcription elongation fa... | NM_001367790.1 | 99.8% | 100% | 6A>G |
| 2 | human | 85012 | TCEAL3 | transcription elongation fa... | NM_001006933.1 | 97.8% | 96.5% | (many diffs) |
| 3 | human | 85012 | TCEAL3 | transcription elongation fa... | NM_032926.3 | 97.8% | 96.5% | (many diffs) |
| 4 | human | 158931 | TCEAL6 | transcription elongation fa... | NM_001006938.3 | 91.3% | 89% | 6A>G;520_521insC;549_550ins50 |
| 5 | human | 340543 | TCEAL5 | transcription elongation fa... | NM_001012979.3 | 89.7% | 86.4% | (many diffs) |
Sequence Information
Note: uppercase bases indicate empirically verified sequence.
- ORF start:
- 69
- ORF end:
- 669
- ORF length:
- 600
- Sequence:
-
1 ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag 61 ttggcaccat ggagaaaccc tacaataaaa atgaaggaaa cctggaaaac gagggaaagc 121 cagaagatga agtagagcct gatgatgaag gaaagtcaga cgaggaagaa aagccagacg 181 cggaggggaa gacagaatgc gagggaaagc gaaaggctga gggagagcca ggtgatgagg 241 gacaactgga agataaggga agccaggaaa agcagggcaa gtccgaaggt gagggcaagc 301 cacaaggcga gggcaagcca gcctcccagg caaagccaga gggccagccg cgggccgccg 361 aaaagcgccc ggctggagat tatgtgcccc ggaaagcaaa aagaaaaaca gacaggggga 421 cggacgattc ccccaaggac tctcaggagg acttacagga aaggcatctg agcagtgagg 481 agatgatgag agaatgtgga gatgtgtcaa gggctcagga ggagctaagg aaaaaacaga 541 aaatgggtgg ttttcattgg atgcaaagag atgtacagga tccattcgcc ccaaggggac 601 aacggggtgt caggggagtg aggggtggag gtaggggcca aagaggctta cacgatatcc 661 catacctttt gcaactttct tgtacaaagt ggttggtaag cctatcccta accctctcct 721 cggtctcgat tctacgtagt aatgagctag cgctaaccgg tggcgcgtta agtcgacaat 781 caacctctgg atta