Transcript: Human NM_001367790.1

Homo sapiens transcription elongation factor A like 6 (TCEAL6), transcript variant 1, mRNA.

Source:
NCBI, updated 2019-08-08
Taxon:
Homo sapiens (human)
Gene:
TCEAL6 (158931)
Length:
3057
CDS:
227..829

Additional Resources:

NCBI RefSeq record:
NM_001367790.1
NBCI Gene record:
TCEAL6 (158931)

sgRNA constructs matching this transcript (CRISPRko, NGG PAM)

This list includes CRISPRko constructs with 100% (20mer + NGG) sequence match to the coding regions of this transcript.

No results found.

shRNA constructs matching this transcript with 100% SDR[?] match

This list includes all shRNAs that have a perfect SDR[?] match to Human NM_001367790.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon).

Clone ID Target Seq Vector Match Position Match Region[?] SDR Match %[?] Intrinsic Score[?] Adjusted Score[?] Matches Other Human Gene?[?] Orig. Target Gene[?] Addgene[?]
1 TRCN0000163651 GCCAAAGAGGCTTACACGATA pLKO.1 795 CDS 100% 4.950 3.465 N TCEAL6 n/a
2 TRCN0000160833 GACAACTGGAAGATAAGGGAA pLKO.1 399 CDS 100% 2.640 1.584 N TCEAL6 n/a
3 TRCN0000163443 GAGGCTTACACGATATCCCAT pLKO.1 801 CDS 100% 2.640 1.584 N TCEAL6 n/a
4 TRCN0000159694 GCTTTAGATGTCAATCTCGTT pLKO.1 937 3UTR 100% 2.640 1.584 N TCEAL6 n/a
5 TRCN0000158676 GCTTTAACCTTAAGCTGATAT pLKO.1 912 3UTR 100% 13.200 6.600 Y TCEAL6 n/a
6 TRCN0000135939 GAAAGTCAGACGAGGAAGAAA pLKO.1 309 CDS 100% 5.625 2.813 Y TCEAL3 n/a
7 TRCN0000143304 GAAAGTCAGACGAGGAAGAAA pLKO.1 309 CDS 100% 5.625 2.813 Y TCEAL5 n/a
8 TRCN0000134788 CATTGGATGCAAAGAGATGTA pLKO.1 713 CDS 100% 4.950 2.475 Y TCEAL3 n/a
9 TRCN0000135361 GAGAATGTGGAGATGTGTCAA pLKO.1 648 CDS 100% 4.950 2.475 Y TCEAL3 n/a
10 TRCN0000143539 GAGAATGTGGAGATGTGTCAA pLKO.1 648 CDS 100% 4.950 2.475 Y TCEAL5 n/a
11 TRCN0000163749 GAGGGAAAGCCAGAAGATGAA pLKO.1 269 CDS 100% 4.950 2.475 Y TCEAL6 n/a
12 TRCN0000135975 GATGAAGTAGAGCCTGATGAT pLKO.1 284 CDS 100% 4.950 2.475 Y TCEAL3 n/a
13 TRCN0000161863 GATGAGAGAATGTGGAGATGT pLKO.1 643 CDS 100% 4.950 2.475 Y TCEAL6 n/a
14 TRCN0000137398 GATGAGGGACAACTGGAAGAT pLKO.1 392 CDS 100% 4.950 2.475 Y TCEAL3 n/a
15 TRCN0000139879 GATGAGGGACAACTGGAAGAT pLKO.1 392 CDS 100% 4.950 2.475 Y TCEAL5 n/a
16 TRCN0000135402 GTGAGGAGATGATGAGAGAAT pLKO.1 633 CDS 100% 4.950 2.475 Y TCEAL3 n/a
17 TRCN0000140543 GAAGACAGAATGCGAGGGAAA pLKO.1 346 CDS 100% 4.050 2.025 Y TCEAL5 n/a
18 TRCN0000163356 GAAGACAGAATGCGAGGGAAA pLKO.1 346 CDS 100% 4.050 2.025 Y TCEAL6 n/a
19 TRCN0000135603 GAAGATGAAGTAGAGCCTGAT pLKO.1 281 CDS 100% 4.050 2.025 Y TCEAL3 n/a
20 TRCN0000135336 CCTGATGATGAAGGAAAGTCA pLKO.1 296 CDS 100% 3.000 1.500 Y TCEAL3 n/a
21 TRCN0000135813 CAAAGAGATGTACAGGATCCA pLKO.1 722 CDS 100% 2.640 1.320 Y TCEAL3 n/a
22 TRCN0000163115 GAAGGAAAGTCAGACGAGGAA pLKO.1 305 CDS 100% 2.640 1.320 Y TCEAL6 n/a
23 TRCN0000161028 GAGATGATGAGAGAATGTGGA pLKO.1 638 CDS 100% 2.640 1.320 Y TCEAL6 n/a
24 TRCN0000137218 GAGGACTTACAGGAAAGGCAT pLKO.1 605 CDS 100% 2.640 1.320 Y TCEAL3 n/a
25 TRCN0000162766 CCAGAAGATGAAGTAGAGCCT pLKO.1 278 CDS 100% 0.660 0.330 Y TCEAL6 n/a
26 TRCN0000179624 GAAGATGAAGTAGAGCCTGAA pLKO.1 281 CDS 100% 4.050 2.025 Y Tceal6 n/a
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shRNA constructs with at least a near match to this transcript

This list includes shRNAs that have at least a >84% (16 of 19 bases) SDR[?] match to the transcript NM_001367790.1, regardless of what transcript they were originally designed to target. For example, this list can include shRNAs that were originally designed to target: (i) a different isoform or obsolete version of this transcript (as annotated by NCBI), (ii) a transcript of an orthologous gene (in this collection, generally human-to-mouse or mouse-to-human), or (iii) a transcript of a different gene (from the same or different taxon). NOTE: this download is a superset of the above result set.

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All ORF constructs matching this transcript

Clone ID DNA Barcode Vector Sequenced %[?] Nuc. Match %[?] Prot. Match %[?] Epitope Tag Match Diffs[?] Addgene[?]
1 ccsbBroadEn_14415 pDONR223 100% 99.8% 100% None 6A>G n/a
2 ccsbBroad304_14415 pLX_304 0% 99.8% 100% V5 (not translated due to frame shift) 6A>G n/a
3 ccsbBroadEn_04467 pDONR223 100% 98% 96.5% None (many diffs) n/a
4 ccsbBroad304_04467 pLX_304 0% 98% 96.5% V5 (many diffs) n/a
5 TRCN0000473638 CTGTTCTTGGGGTCCTCCGGTTAT pLX_317 86.9% 98% 96.5% V5 (many diffs) n/a
6 ccsbBroadEn_05474 pDONR223 100% 89.8% 86.4% None (many diffs) n/a
7 ccsbBroad304_05474 pLX_304 0% 89.8% 86.4% V5 (many diffs) n/a
8 TRCN0000480304 TAATTTTTACCGTGGCATATTACT pLX_317 61.7% 89.8% 86.4% V5 (many diffs) n/a
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