Construct: ORF ccsbBroad304_14431

Construct Description:

Construct Type:
ORF
Other Identifiers:
ORF006021.1_s304c1, BRDN0000409116
Derived from:
ccsbBroadEn_14431
DNA Barcode:
None
Epitope Tag:
V5 (not translated due to frame shift)
Notes:
No stop codon in insert

Originally Annotated References:

Gene:
NAIF1 (203245)

Vector Information:

Vector Backbone:
pLX_304
Pol II Cassette 1:
PGK-BlastR
Pol II Cassette 2:
CMV-ccsbBroad304_14431
Selection Marker:
BlastR
Visible Reporter:
n/a
Epitope Tag:
V5

Current transcripts matched by this ORF:

Taxon Gene Symbol Description Transcript Nuc. Match %[?] Prot. Match %[?] Match Diffs[?]
1 human 203245 NAIF1 nuclear apoptosis inducing ... NM_197956.4 52.1% 51.9% (many diffs)
2 human 203245 NAIF1 nuclear apoptosis inducing ... XR_242574.2 21.3% (many diffs)
3 human 203245 NAIF1 nuclear apoptosis inducing ... XR_428519.3 13.2% (many diffs)
4 mouse 71254 Naif1 nuclear apoptosis inducing ... NM_194335.2 46.3% 49.5% (many diffs)
Download CSV

Sequence Information

Note: uppercase bases indicate empirically verified sequence.

ORF start:
66
ORF end:
582
ORF length:
516
Sequence:
1ggtctatata agcagagctc tctggctaac tgtcgggatc aacaagtttg tacaaaaaag
61ttggcatggc cgtaccagcc aagaaaagga agatgaactt ctcagagcgg gaggtggaga
121tcatcgtgga ggagctggag ctgaagaagc acctgctggt gaaccacttc aacgccgggg
181tacccctggc cgccaagagt gcggcctggc acggcatcct gagaagggtc aacgccgtgg
241ccacctgccg cagagagctg cctgaggtca agaagaagtg gtctgacctc aagaccgagg
301tccgtcgcaa ggttgcccag gtccgggccg ccgtggaggg tggtgaggcg ccggggccca
361ctgaggagga cggagctggg gggcctggga caggcggtgg cagtggcggc ggtggcccag
421ctgtagcccc agtgctgctg acccccatgc aacaacgtat ctgcaacctg ctgggcgagg
481ccaccatcat cagcctgccc agcaccacag agatccaccc tgtggccctc ggaccctcgg
541ccaccgcagc cgcagccacg gtcaccctga cacagagtag tgccccactt tcttgtacaa
601agtggttggt aagcctatcc ctaaccctct cctcggtctc gattctacgt agtaatgagc
661tagcgctaac cggtggcgcg ttaagtcgac aatcaacctc tggatta

Download FASTA (ORF) (Full)